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Occurrence of all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3) in roughscale sole Clidoderma asperrimum flesh lipids
| Title: | Occurrence of all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3) in roughscale sole Clidoderma asperrimum flesh lipids |
| Other Titles: | サメガレイClidoderma asperrimum の筋肉脂質における全-cis-5,8,11,14,17,20,23-ヘキサコサヘプタエン酸（26:7n-3）の存在 |
| Authors: | Fukuda, Yuuki1 Browse this author | | Ando, Yasuhiro2 Browse this author →KAKEN DB |
| Authors(alt): | 福田, 悠紀1 | | 安藤, 靖浩2 |
| Keywords: | Hexacosaheptaenoic acid | | Very long chain polyunsaturated fatty acid | | Fatty acid | | Roughscale sole | | Clidoderma asperrimum | | GC | | GC–MS |
| Issue Date: | Sep-2011 |
| Publisher: | Japanese Society of Fisheries Science |
| Journal Title: | Fisheries Science |
| Volume: | 77 |
| Issue: | 5 |
| Start Page: | 875 |
| End Page: | 882 |
| Publisher DOI: | 10.1007/s12562-011-0387-y |
| Abstract: | Fatty acid analysis of roughscale sole Clidoderma asperrimum flesh lipids was carried out by gas chromatography. An unidentified peak appeared in the chromatogram in the elution region of ≥C24 fatty acids. After enrichment by solvent partitioning, reversed-phase thin-layer chromatography (TLC), and argentation TLC, the peak component was subjected to structural analyses. The partially hydrogenated products after reaction with hydrazine hydrate gave seven isomers of cis-hexacosenoic acid (26:1). Gas chromatography-mass spectrometry (GC–MS) analyses of their dimethyl disulfide adducts identified the monounsaturates as 5-, 8-, 11-, 14-, 17-, 20-, and 23-26:1. The peak component was assigned to all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3). GC–MS analyses of the 4,4-dimethyloxazoline derivative and methyl ester confirmed this structure. This fatty acid is a rare, very long-chain polyunsaturated fatty acid (VLCPUFA). The concentrations of the acid found in roughscale sole were 0.69 ± 0.34% (N = 5) of total fatty acids in flesh lipids. Roughscale sole appears to be characterized by the occurrence of 26:7n-3, which is lacking in popular sources of methylene-interrupted VLCPUFA, such as vertebrate retina, spermatozoa, and herring. | | 北海道産サメガレイの筋肉脂質の脂肪酸をGC で分析したところ，炭素数24 以上の領域に未知のピークが出現した。この成分を濃縮後，数種の誘導体に変換してGC-MSに供した結果，同成分は全-cis-5,8,11,14,17,20,23-ヘキサコサヘプタエン酸（26:7n-3）と同定された。総脂肪酸中の含有量は供試5 個体の平均で0.69±0.34％であった。サメガレイは，n-3 系およびn-6 系超長鎖ポリエン酸の給源として一般的な高等動物の網膜，精子，ニシン油などには見られない26:7n-3 を含む点で特徴的である。 |
| Rights: | © 2011 公益社団法人日本水産学会 | | © 2011 The Japanese Society of Fisheries Science | | The final publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/47167 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 安藤 靖浩
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