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A20 Silencing by Lipid Envelope-Type Nanoparticles Enhances the Efficiency of Lipopolysaccharide-Activated Dendritic Cells
| Title: | A20 Silencing by Lipid Envelope-Type Nanoparticles Enhances the Efficiency of Lipopolysaccharide-Activated Dendritic Cells |
| Authors: | Warashina, Shota Browse this author | | Nakamura, Takashi Browse this author | | Harashima, Hideyoshi Browse this author |
| Keywords: | non-viral delivery system | | short interference RNA | | dendritic cell | | A20 | | multifunctional envelope-type nano device |
| Issue Date: | Aug-2011 |
| Publisher: | Pharmaceutical Society of Japan |
| Journal Title: | Biological & Pharmaceutical Bulletin |
| Volume: | 34 |
| Issue: | 8 |
| Start Page: | 1348 |
| End Page: | 1351 |
| Publisher DOI: | 10.1248/bpb.34.1348 |
| Abstract: | In a previous report, we described the development of lipid envelope-type nanoparticles (MEND) modified with octaarginine (R8) and a pH-sensitive fusogenic peptide (GALA) for delivering short interference RNA (siRNA) to mouse dendritic cells (DCs). A20 was recently reported to be a negative regulator of the toll-like receptor and the tumor necrosis factor receptor signaling pathways. Although A20 would be expected to be a useful target for boosting the effects of adjuvants in DC immunotherapy, limited information is available regarding the use of A20-silenced DC by an original non-viral vector. In this study, we loaded anti-A20 siRNA into a MEND and investigated the gene knockdown activity in DC and the immunological functions of A20-silenced DC. The use of a MEND resulted in a significant A20 knockdown effect, and the A20-silenced DC resulted in an enhanced production of proinflammatory molecules, after lipopolysaccharide (LPS) stimulation. The expression of co-stimulatory molecules by LPS stimulation was also increased in the A20-silenced DC. The findings reported herein show that a MEND loaded with anti-A20 siRNA is a potent non-viral vector that has the ability to enhance the adjuvant effect of LPS in DC. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/47203 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 中村 孝司
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