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Biochemical characterization of the very long-chain fatty acid elongase ELOVL7

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Title: Biochemical characterization of the very long-chain fatty acid elongase ELOVL7
Authors: Naganuma, Tatsuro Browse this author
Sato, Yuichiro Browse this author
Sassa, Takayuki Browse this author
Ohno, Yusuke Browse this author
Kihara, Akio Browse this author →KAKEN DB
Keywords: Very long-chain fatty acid
Elongase
Lipid
ELOVL
Membrane
Issue Date: 20-Oct-2011
Publisher: Elsevier B.V.
Journal Title: FEBS Letters
Volume: 585
Issue: 20
Start Page: 3337
End Page: 3341
Publisher DOI: 10.1016/j.febslet.2011.09.024
PMID: 21959040
Abstract: Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we purified ELOVL7 and measured its activity by reconstituting it into proteoliposomes. Purified ELOVL7 exhibited high activity toward acyl-CoAs with C18 carbon chain length. The calculated Km values toward C18: 3-CoA(n-3) and malonyl-CoA were both in the μM range. We also found that progression of the VLCFA cycle enhances ELOVL7 activity.
Type: article (author version)
URI: http://hdl.handle.net/2115/47466
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木原 章雄

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