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Gene expression profile of the cartilage tissue spontaneously regenerated in vivo by using a novel double-network gel: Comparisons with the normal articular cartilage

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/47497

Title: Gene expression profile of the cartilage tissue spontaneously regenerated in vivo by using a novel double-network gel: Comparisons with the normal articular cartilage
Authors: Imabuchi, Ryusei Browse this author
Ohmiya, Yoshihiro Browse this author
Joon Kwon, Hyuck Browse this author
Onodera, Shin Browse this author →KAKEN DB
Kitamura, Nobuto Browse this author →KAKEN DB
Kurokawa, Takayuki Browse this author →KAKEN DB
Gong, Jian Ping Browse this author →KAKEN DB
Yasuda, Kazunori Browse this author →KAKEN DB
Issue Date: 2011
Publisher: BioMed Central
Journal Title: BMC Musculoskeletal Disorders
Volume: 12
Issue: 1
Start Page: 213
End Page: 224
Publisher DOI: 10.1186/1471-2474-12-213
Abstract: Background: We have recently found a phenomenon that spontaneous regeneration of a hyaline cartilage-liketissue can be induced in a large osteochondral defect by implanting a double-network (DN) hydrogel plug, whichwas composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N, N’-Dimetyl acrylamide), at thebottom of the defect. The purpose of this study was to clarify gene expression profile of the regenerated tissue incomparison with that of the normal articular cartilage. Methods: We created a cylindrical osteochondral defect in the rabbit femoral grooves. Then, we implanted the DNgel plug at the bottom of the defect. At 2 and 4 weeks after surgery, the regenerated tissue was analyzed usingDNA microarray and immunohistochemical examinations. Results: The gene expression profiles of the regenerated tissues were macroscopically similar to the normalcartilage, but showed some minor differences. The expression degree of COL2A1, COL1A2, COL10A1, DCN, FMOD,SPARC, FLOD2, CHAD, CTGF, and COMP genes was greater in the regenerated tissue than in the normal cartilage.The top 30 genes that expressed 5 times or more in the regenerated tissue as compared with the normal cartilageincluded type-2 collagen, type-10 collagen, FN, vimentin, COMP, EF1alpha, TFCP2, and GAPDH genes. Conclusions: The tissue regenerated by using the DN gel was genetically similar but not completely identical toarticular cartilage. The genetic data shown in this study are useful for future studies to identify specific genesinvolved in spontaneous cartilage regeneration.
Rights: http://creativecommons.org/licenses/by/2.0/
Type: article
URI: http://hdl.handle.net/2115/47497
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 龔 剣萍 (Gong Jian Ping)

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