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Establishment of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction of β-oxidation in Escherichia coli LS5218
| Title: | Establishment of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction of β-oxidation in Escherichia coli LS5218 |
| Authors: | Shozui, Fumi Browse this author | | Matsumoto, Ken'ichiro Browse this author →KAKEN DB | | Motohashi, Ren Browse this author | | Yamada, Miwa Browse this author | | Taguchi, Seiichi Browse this author →KAKEN DB |
| Keywords: | Poly(LA-co-3HB-co-3HHx) | | LA-based polyester | | PhaC1_[Ps](ST/QK) | | Microbial cell factory | | Metabolic engineering | | β-oxidation pathway |
| Issue Date: | Aug-2010 |
| Publisher: | Elsevier |
| Journal Title: | Polymer Degradation and Stability |
| Volume: | 95 |
| Issue: | 8 |
| Start Page: | 1340 |
| End Page: | 1344 |
| Publisher DOI: | 10.1016/j.polymdegradstab.2010.01.029 |
| Abstract: | New lactate (LA)-based terpolymers, P[LA-co-3-hydroxybutyrate (3HB)-co-3-hydroxyhexananoate (3HHx)]s, were produced in recombinant Escherichia coli LS5218 harboring three genes encoding LA-polymerizing enzyme (LPE), propionyl-coenzyme A (CoA) transferase (PCT) and (R)-specific enoyl-CoA hydratase (PhaJ4). When the recombinant LS5218 was grown on glucose with the feeding of butyrate, 3HB-CoA and 3HHx-CoA were supplied, probably via reverse reactions of the β-oxidation pathway and PhaJ4. LPE copolymerized the two monomers with LA-CoA, which was generated by PCT, to yield the terpolymers. Gas chromatography analysis revealed that the terpolymers consisted of 2.7 to 34 mol% LA, 38 to 81 mol% 3HB and 17 to 33 mol% 3HHx units, which can be varied depending on the butyrate concentration fed in the medium. In addition, 1H-13C COSY NMR analysis provided evidence for a linkage between LA and 3HHx units in the polymer. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/47513 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松本 謙一郎
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