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Norovirus-binding proteins recovered from activated sludge micro-organisms with an affinity to a noroviral capsid peptide

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Title: Norovirus-binding proteins recovered from activated sludge micro-organisms with an affinity to a noroviral capsid peptide
Authors: Sano, Daisuke Browse this author →KAKEN DB
Wada, Keishi Browse this author
Imai, Takahiro Browse this author
Masago, Yoshifumi Browse this author
Omura, Tatsuo Browse this author
Keywords: activated sludge micro-organisms
affinity chromatography
hydrophobic interaction
virus-binding protein
virus-like particle
Issue Date: Dec-2010
Publisher: Blackwell Publishing
Journal Title: Journal of Applied Microbiology
Volume: 109
Issue: 6
Start Page: 1923
End Page: 1928
Publisher DOI: 10.1111/j.1365-2672.2010.04821.x
PMID: 20735509
Abstract: Aims: Transmission routes of noroviruses, leading aetiological agents of acute gastroenteritis, are rarely verified when outbreaks occur. Since the destination of norovirus particles being firmly captured by microorganisms could be totally different from that of those particles moving freely, microorganisms with natural affinity ligands such as virus-binding proteins would affect the fate of viruses in environment, if such microbial affinity ligands exist. The aim of this study is to identify norovirus-binding proteins (NoVBPs) that are presumably working as natural ligands for norovirus particles in water environments. Methods and Results: NoVBPs were recovered from activated sludge microorganisms by an affinity chromatography technique in which a capsid peptide of norovirus genogroup II (GII) was immobilized. The recovered NoVBPs bind to norovirus-like particles (NoVLPs) of norovirus GII, and this adsorption was stronger than that to NoVLPs of norovirus genogroup I. The profile of two-dimensional electrophoresis of NoVBPs showed that the recovered NoVBPs included at least 7 spots of protein. The determination of N-terminal amino acid sequences of these NoVBPs revealed that hydrophobic interactions could contribute to the adsorption between NoVBPs and norovirus particles. Conclusions: NoVBPs conferring a high affinity to norovirus GII were successfully isolated from activated sludge microorganisms. Significance and Impact of the Study: NoVBPs could be natural viral ligands and play an important role in the NoV transmission.
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Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐野 大輔

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