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Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase

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タイトル: Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase
著者: Matsumoto, Ken'ichiro 著作を一覧する
Ishiyama, Ayako 著作を一覧する
Sakai, Kohei 著作を一覧する
Shiba, Tetsufumi 著作を一覧する
Taguchi, Seiichi 著作を一覧する
キーワード: Poly(glycolide)
Poly(lactide)
Lactate-polymerizing enzyme
Biobased plastic
Polyhydroxyalkanoate
発行日: 2011年12月10日
出版者: Elsevier B.V.
誌名: Journal of Biotechnology
巻: 156
号: 3
開始ページ: 214
終了ページ: 217
出版社 DOI: 10.1016/j.jbiotec.2011.07.040
抄録: Glycolate(GL)-based polyesters were for the first time produced in the recombinant Escherichia coli fatty acid β-oxidation pathway reinforcing mutant LS5218, using extracellularly added GL as a monomer precursor. Cells expressing a Ser325Thr/Gln481Lys mutant of polyhydroxyalkanoate synthase (PhaC1STQK) from Pseudomonas sp. 61-3, propionyl-CoA transferase from Megasphaera elsdenii and enoyl-CoA hydratase from Pseudomonas aeruginosa grown on GL and dodecanoate were found to produce novel copolymers of GL with 3-hydroxyalkanoates (3HAs) (C4-C12), P(GL-co-3HA), with a weight-average molecular weight of 34000. The 1H NMR analyses of the copolymer revealed the incorporation of GL units into the polymer chain. This result demonstrates that PhaC1STQK polymerized glycolyl-CoA as a monomer substrate. Additionally, the novel lactate(LA)-based polyester P(LA-co-3HA) was produced by substituting GL with LA, indicating that the method is versatile and allows the production of a variety of biopolymers.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/47658
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 松本 謙一郎

 

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