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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase
| Title: | Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase |
| Authors: | Matsumoto, Ken'ichiro Browse this author →KAKEN DB | | Ishiyama, Ayako Browse this author | | Sakai, Kohei Browse this author | | Shiba, Tetsufumi Browse this author | | Taguchi, Seiichi Browse this author →KAKEN DB |
| Keywords: | Poly(glycolide) | | Poly(lactide) | | Lactate-polymerizing enzyme | | Biobased plastic | | Polyhydroxyalkanoate |
| Issue Date: | 10-Dec-2011 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Journal of Biotechnology |
| Volume: | 156 |
| Issue: | 3 |
| Start Page: | 214 |
| End Page: | 217 |
| Publisher DOI: | 10.1016/j.jbiotec.2011.07.040 |
| PMID: | 21907740 |
| Abstract: | Glycolate(GL)-based polyesters were for the first time produced in the recombinant Escherichia coli fatty acid β-oxidation pathway reinforcing mutant LS5218, using extracellularly added GL as a monomer precursor. Cells expressing a Ser325Thr/Gln481Lys mutant of polyhydroxyalkanoate synthase (PhaC1STQK) from Pseudomonas sp. 61-3, propionyl-CoA transferase from Megasphaera elsdenii and enoyl-CoA hydratase from Pseudomonas aeruginosa grown on GL and dodecanoate were found to produce novel copolymers of GL with 3-hydroxyalkanoates (3HAs) (C4-C12), P(GL-co-3HA), with a weight-average molecular weight of 34000. The 1H NMR analyses of the copolymer revealed the incorporation of GL units into the polymer chain. This result demonstrates that PhaC1STQK polymerized glycolyl-CoA as a monomer substrate. Additionally, the novel lactate(LA)-based polyester P(LA-co-3HA) was produced by substituting GL with LA, indicating that the method is versatile and allows the production of a variety of biopolymers. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/47658 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松本 謙一郎
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