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Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.)

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Title: Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.)
Authors: Wang, Xun Browse this author
Hoshino, Yoichiro Browse this author →KAKEN DB
Yamada, Toshihiko Browse this author →KAKEN DB
Keywords: Gibberellic acid (GA3)
in vitro culture
mature embryos
thidiazuron (TDZ)
Issue Date: Dec-2010
Publisher: Wiley-Blackwell
Journal Title: Grassland Science
Volume: 56
Issue: 4
Start Page: 198
End Page: 204
Publisher DOI: 10.1111/j.1744-697X.2010.00195.x
Abstract: A rapid and efficient in vitro culture system has been established in zoysiagrass (Zoysia japonica Steud.). Embryos isolated from mature seeds were used to induce callus, which improved callus formation and shortened the culture period. A high concentration of 2,4-dichlorophenoxyacetic acid (5 mg L−1) in combination with low concentration of 6-benzyladenine (0.2 mg L−1) was proved to be suitable for callus induction in zoysiagrass. Eight types of calli were observed; friable callus was the most regenerable type. Shoot regeneration efficiency was improved by using 1 mg L−1 of thidiazuron with 1-naphthaleneacetic acid (0.05 mg L−1) and gibberellic acid (0.1 mg L−1). Zoysiagrass shoots continued to grow in MS medium, and rooted in half-strength MS medium. With this system, it appears the duration of in vitro regeneration could be shortened to 16 weeks for zoysiagrass, i.e. from inoculation of embryos to regeneration of plantlets with shoots and roots.
Rights: The definitive version is available at
Type: article (author version)
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 敏彦

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