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Mitochondrial delivery of mastoparan with transferrin liposomes equipped with a pH-sensitive fusogenic peptide for selective cancer therapy.
Title: | Mitochondrial delivery of mastoparan with transferrin liposomes equipped with a pH-sensitive fusogenic peptide for selective cancer therapy. |
Authors: | Yamada, Yuma Browse this author | Shinohara, Yasuo Browse this author | Kakudo, Tomoyuki Browse this author | Chaki, Shinji Browse this author | Futaki, Shiroh Browse this author | Kamiya, Hiroyuki Browse this author | Harashima, Hideyoshi Browse this author |
Keywords: | mastoparan | transferrrin liposomes | GALA | mitochondrial delivery | anticancer therapy | apoptosis |
Issue Date: | 13-Oct-2005 |
Publisher: | Elsevier |
Journal Title: | International journal of pharmaceutics |
Volume: | 303 |
Issue: | 1-2 |
Start Page: | 1 |
End Page: | 7 |
Publisher DOI: | 10.1016/j.ijpharm.2005.06.009 |
PMID: | 16139452 |
Abstract: | Mastoparan (MP), a potent facilitator of mitochondrial permeability transition (PT), could be used as an antitumor agent, if it were encapsulated in a tumor selective delivery system. We recently developed transferrin-modified liposomes (Tf-L) with a pH-sensitive fusogenic peptide (GALA), which delivers an encapsulated fluorescent marker into cytosol efficiently. In this study, we encapsulated MP into Tf-L with GALA for the selective delivery to mitochondria of tumor cells. The MP showed potent PT activity at concentrations above 25 microM in a homogenate of K 562 cells as well as in isolated mitochondria in the presence of phosphate. Tf-L equipped with cholesteryl GALA can release encapsulated sulforhodamine B, while Tf-L failed, as evidenced by confocal laser scanning microscopy. The MP, which was delivered with Tf-L with GALA, released cytochrome c (cyt c) from mitochondria to the cytosol, while free MP released cyt c not only to the cytosol but also extracellulary. These results demonstrate the utility of MP in Tf-L with GALA for cancer therapy. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/48155 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 勇磨
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