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In silico and in vivo identification of the intermediate filament vimentin that is downregulated downstream of Brachyury during Xenopus embryogenesis

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/48198

Title: In silico and in vivo identification of the intermediate filament vimentin that is downregulated downstream of Brachyury during Xenopus embryogenesis
Authors: Yamada, Atsuko Browse this author
Koyanagi, Kanako O. Browse this author
Watanabe, Hidemi Browse this author
Keywords: T/brachyury
T site
Cell movement
Clawed frog
Vimentin
Issue Date: 10-Jan-2012
Publisher: Elsevier B.V.
Journal Title: Gene
Volume: 491
Issue: 2
Start Page: 232
End Page: 236
Publisher DOI: 10.1016/j.gene.2011.09.007
PMID: 21963995
Abstract: Brachyury, a member of the T-box transcription family, has been suggested to be essential for morphogenetic movements in various processes of animal development. However, little is known about its critical transcriptional targets. In order to identify targets of Brachyury and understand the molecular mechanisms underlying morphogenetic movements, we first searched the genome sequence of Xenopus tropicalis, the only amphibian genomic sequence available, for Brachyury-binding sequences known as T-half sites, and then screened for the ones conserved between vertebrate genomes. We found three genes that have evolutionarily conserved T-half sites in the promoter regions and examined these genes experimentally to determine whether their expressions were regulated by Brachyury, using the animal cap system of Xenopus laevis embryos. Eventually, we obtained evidence that vimentin, encoding an intermediate filament protein, was a potential target of Brachyury. This is the first report to demonstrate that Brachyury might affect the cytoskeletal structure through regulating the expression of an intermediate filament protein, vimentin.
Type: article (author version)
URI: http://hdl.handle.net/2115/48198
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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