HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
情報科学研究科  >
雑誌発表論文等  >

In silico and in vivo identification of the intermediate filament vimentin that is downregulated downstream of Brachyury during Xenopus embryogenesis

フルテキスト
Supplementary_material_2.xlsxSupplementary material285.9 kBMicrosoft Excel XML見る/開く
Gen491-2_232-236.pdf456.62 kBPDF見る/開く
Supplementary_material_1.docSupplementary material2.88 MBMicrosoft Word見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/48198

タイトル: In silico and in vivo identification of the intermediate filament vimentin that is downregulated downstream of Brachyury during Xenopus embryogenesis
著者: Yamada, Atsuko 著作を一覧する
Koyanagi, Kanako O. 著作を一覧する
Watanabe, Hidemi 著作を一覧する
キーワード: T/brachyury
T site
Cell movement
Clawed frog
Vimentin
発行日: 2012年 1月10日
出版者: Elsevier B.V.
誌名: Gene
巻: 491
号: 2
開始ページ: 232
終了ページ: 236
出版社 DOI: 10.1016/j.gene.2011.09.007
抄録: Brachyury, a member of the T-box transcription family, has been suggested to be essential for morphogenetic movements in various processes of animal development. However, little is known about its critical transcriptional targets. In order to identify targets of Brachyury and understand the molecular mechanisms underlying morphogenetic movements, we first searched the genome sequence of Xenopus tropicalis, the only amphibian genomic sequence available, for Brachyury-binding sequences known as T-half sites, and then screened for the ones conserved between vertebrate genomes. We found three genes that have evolutionarily conserved T-half sites in the promoter regions and examined these genes experimentally to determine whether their expressions were regulated by Brachyury, using the animal cap system of Xenopus laevis embryos. Eventually, we obtained evidence that vimentin, encoding an intermediate filament protein, was a potential target of Brachyury. This is the first report to demonstrate that Brachyury might affect the cytoskeletal structure through regulating the expression of an intermediate filament protein, vimentin.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/48198
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 渡邉 日出海

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学