|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy
| Title: | Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy |
| Authors: | Okada, Akinori Browse this author | | Mizutani, Yusuke Browse this author | | Subagyo, Agus Browse this author | | Hosoi, Hirotaka Browse this author | | Nakamura, Motonori Browse this author | | Sueoka, Kazuhisa Browse this author | | Kawahara, Koichi Browse this author | | Okajima, Takaharu Browse this author →KAKEN DB |
| Keywords: | adhesion | | atomic force microscopy | | biological techniques | | biomechanics | | cellular biophysics |
| Issue Date: | 26-Dec-2011 |
| Publisher: | American Institute of Physics |
| Journal Title: | Applied Physics Letters |
| Volume: | 99 |
| Issue: | 26 |
| Start Page: | 263703 |
| Publisher DOI: | 10.1063/1.3672225 |
| Abstract: | We investigated dynamic force propagation between focal adhesions of fibroblast cells cultured on polydimethylsiloxane micropost substrates, by atomic force microscopy. Live cells were mechanically modulated by the atomic force microscopy probe bound to cell apical surfaces at 0.01-0.5 Hz, while microposts served as a force sensor at basal surfaces. We observed that cells exhibited rheological behavior at the apical surface but had no apparent out-of-phase response at the basal surface, indicating that the dynamic force propagating through cytoskeletal filaments behaves in an elastic manner. Moreover, the direction of the propagated force was observed to be intimately associated with the prestress. |
| Rights: | Copyright 2011 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 99, 263703 (2011) and may be found at https://dx.doi.org/10.1063/1.3672225 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/48293 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 岡嶋 孝治
|
