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Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy

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Title: Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy
Authors: Okada, Akinori Browse this author
Mizutani, Yusuke Browse this author
Subagyo, Agus Browse this author
Hosoi, Hirotaka Browse this author
Nakamura, Motonori Browse this author
Sueoka, Kazuhisa Browse this author
Kawahara, Koichi Browse this author
Okajima, Takaharu Browse this author →KAKEN DB
Keywords: adhesion
atomic force microscopy
biological techniques
biomechanics
cellular biophysics
Issue Date: 26-Dec-2011
Publisher: American Institute of Physics
Journal Title: Applied Physics Letters
Volume: 99
Issue: 26
Start Page: 263703
Publisher DOI: 10.1063/1.3672225
Abstract: We investigated dynamic force propagation between focal adhesions of fibroblast cells cultured on polydimethylsiloxane micropost substrates, by atomic force microscopy. Live cells were mechanically modulated by the atomic force microscopy probe bound to cell apical surfaces at 0.01-0.5 Hz, while microposts served as a force sensor at basal surfaces. We observed that cells exhibited rheological behavior at the apical surface but had no apparent out-of-phase response at the basal surface, indicating that the dynamic force propagating through cytoskeletal filaments behaves in an elastic manner. Moreover, the direction of the propagated force was observed to be intimately associated with the prestress.
Rights: Copyright 2011 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 99, 263703 (2011) and may be found at https://dx.doi.org/10.1063/1.3672225
Type: article
URI: http://hdl.handle.net/2115/48293
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岡嶋 孝治

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