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CREB-binding protein transcription activation domain for enhanced transgene expression by a positive feedback system

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Title: CREB-binding protein transcription activation domain for enhanced transgene expression by a positive feedback system
Authors: Kanda, Genki Browse this author
Ochiai, Hiroshi Browse this author
Harashima, Hideyoshi Browse this author
Kamiya, Hiroyuki Browse this author →KAKEN DB
Keywords: Artificial transcription factor
Positive feedback system
CREB-binding protein
Issue Date: Jan-2012
Publisher: Elsevier B.V.
Journal Title: Journal of Biotechnology
Volume: 157
Issue: 1
Start Page: 7
End Page: 11
Publisher DOI: 10.1016/j.jbiotec.2011.09.016
PMID: 21963597
Abstract: The positive feedback system using a fusion protein of the sequence-specific DNA binding domain of yeast GAL4 and the transcription activation domain of herpes simplex virus VP16 (GAL4-VP16), in which GAL4-VP16 promotes its own expression as well as that of a reporter gene product, is useful for efficient transgene expression from plasmid DNA. In this study, the transcription activation domains of endogenous proteins, instead of VP16, were fused to the GAL4 DNA binding domain, and the positive feedback systems employing the novel fusion proteins were examined. Plasmid DNAs encoding the transcription factors were introduced into mouse Hepa 1-6 cells by electroporation and lipofection. Among CREB-binding protein (226-460), sterol regulatory element-binding protein-1 (1-140), p53 (1-70), and Med15 (9-73), the CREB-binding protein functioned efficiently as an activator. These results indicated that the GAL4-CREB-binding protein is useful for enhanced transgene expression by the positive feedback system.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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