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Mutagenicity of secondary oxidation products of 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-hydroxy-2'-deoxyguanosine 5'-triphosphate)

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タイトル: Mutagenicity of secondary oxidation products of 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-hydroxy-2'-deoxyguanosine 5'-triphosphate)
著者: Hori, Mika 著作を一覧する
Suzuki, Tetsuya 著作を一覧する
Minakawa, Noriaki 著作を一覧する
Matsuda, Akira 著作を一覧する
Harashima, Hideyoshi 著作を一覧する
Kamiya, Hiroyuki 著作を一覧する
キーワード: Oxidized nucleotide
8-Oxo-7,8-dihydroguanine
8-Hydroxyguanine
Secondary oxidation product
Spiroiminodihydantoin
Guanidinohydantoin
発行日: 2011年 9月 1日
出版者: Elsevier B.V.
誌名: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
巻: 714
号: 1-2
開始ページ: 11
終了ページ: 16
出版社 DOI: 10.1016/j.mrfmmm.2011.05.015
PMID: 21704046
抄録: 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) is oxidized more easily than normal nucleobases, which can produce spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh). These secondary oxidation products of 8-oxo-7,8-dihydroguanine are highly mutagenic when formed within DNA. To evaluate the mutagenicity of the corresponding oxidation products of 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-hydroxy-2'-deoxyguanosine 5'-triphosphate) in the nucleotide pool, Escherichia coli cells deficient in the mutT gene were treated with H2O2, and the induced mutations were analyzed. Moreover, the 2'-deoxyriboside 5'-triphosphate derivatives of Sp and Gh were also introduced into competent E. coli cells. The H2O2 treatment of mutT E. coli cells resulted in increases of G:C → T:A and A:T → T:A mutations. However, the incorporation of exogenous Sp and Gh 2'-deoxyribonucleotides did not significantly increase the mutation frequency. These results suggested that the oxidation product(s) of 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate induces G:C → T:A and A:T → T:A mutations, and that the 2'-deoxyriboside 5'-triphosphate derivatives of Sp and Gh exhibit quite weak mutagenicity, in contrast to the bases in DNA.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/48349
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 紙谷 浩之

 

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