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Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)

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Title: Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)
Authors: Nagasato, Chikako Browse this author →KAKEN DB
Inoue, Akira Browse this author →KAKEN DB
Mizuno, Masashi Browse this author
Kanazawa, Kazuki Browse this author
Ojima, Takao Browse this author →KAKEN DB
Okuda, Kazuo Browse this author
Motomura, Taizo Browse this author →KAKEN DB
Keywords: brown algae
cell wall
flat cisternae
Golgi derived vesicles
Issue Date: Jul-2010
Publisher: Springer Berlin / Heidelberg
Journal Title: Planta
Volume: 232
Issue: 2
Start Page: 287
End Page: 298
Publisher DOI: 10.1007/s00425-010-1188-8
PMID: 20473516
Abstract: During cytokinesis in brown algal cells, Golgi derived vesicles (GVs) and flat cisternae (FCs) are involved in building the new cell partition membrane. In this study, we followed the membrane fusion process in Silvetia babingtonii zygotes using electron microscopy together with rapid freezing and freeze substitution. After mitosis, many FCs were formed around endoplasmic reticulum clusters and these then spread toward the future cytokinetic plane. Actin depolymerisation using latrunculin B prevented the appearance of the FCs. Fusion of GVs to FCs resulted in structures that were thicker and more elongated (EFCs; expanded flat cisternae). Some complicated membranous structures (MN; membranous network) were formed by interconnection of EFCs and following the arrival of additional GVs. The MN grew into membranous sacs (MSs) as gaps between the MNs disappeared. The MSs were observed in patches along the cytokinetic plane. Neighboring MSs were united to form the new cell partition membrane. An immunocytochemical analysis indicated that fucoidan was synthesized in Golgi bodies and transported by vesicles to the future cytokinetic plane, where the vesicles fused with the FCs. Alginate was not detected until the MS phase. Incubation of sections with cellulase-gold showed that the cellulose content of the new cross wall was not comparable to that of the parent cell wall.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長里 千香子

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