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Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus

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Title: Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus
Authors: Kim, Bo Min Browse this author
Inaba, Jun-ichi Browse this author
Masuta, Chikara Browse this author →KAKEN DB
Keywords: CMV
functional genomics
VIGS
Issue Date: Apr-2011
Publisher: Korean Society for Horticultural Science in co-publication with Springer
Journal Title: Horticulture, Environment, and Biotechnology
Volume: 52
Issue: 2
Start Page: 176
End Page: 182
Publisher DOI: 10.1007/s13580-011-0172-y
Abstract: The Arabidopsis gene AINTEGUMENTA (At-ANT) functions in cell proliferation and organ growth. The ANT protein has two copies of the AP2 domains, R1 and R2. Recently, a partial cDNA sequence of the At-ANT homolog in Antirrhinum majus (Am-ANT) was reported (Delgado-Benarroch et al., 2009). Here, we used virus-induced gene silencing (VIGS) to analyze the function of the reported Am-ANT. We then determine the open reading frame (ORF) of Am-ANT and its predicted amino acid sequence. We induced VIGS using Cucumber mosaic virus (CMV-A1) and suppressed the level of Am-ANT mRNA and noted any phenotypic changes. The function of Am-ANT was very similar to that of At-ANT. The A1:ANT-infected Antirrhinum plants had smaller floral organs and leaves, even though cell sizes were unchanged in flowers and larger in leaves. The CMV-based VIGS showed that the cloned Am-ANT gene was indeed functional in cell proliferation and organ growth as observed for At-ANT. In A. majus, CMV vector provide great advantages for analysis of gene functions.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/48722
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 増田 税

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