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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus
| Title: | Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus |
| Authors: | Kim, Bo Min Browse this author | | Inaba, Jun-ichi Browse this author | | Masuta, Chikara Browse this author →KAKEN DB |
| Keywords: | CMV | | functional genomics | | VIGS |
| Issue Date: | Apr-2011 |
| Publisher: | Korean Society for Horticultural Science in co-publication with Springer |
| Journal Title: | Horticulture, Environment, and Biotechnology |
| Volume: | 52 |
| Issue: | 2 |
| Start Page: | 176 |
| End Page: | 182 |
| Publisher DOI: | 10.1007/s13580-011-0172-y |
| Abstract: | The Arabidopsis gene AINTEGUMENTA (At-ANT) functions in cell proliferation and organ growth. The ANT protein has two copies of the AP2 domains, R1 and R2. Recently, a partial cDNA sequence of the At-ANT homolog in Antirrhinum majus (Am-ANT) was reported (Delgado-Benarroch et al., 2009). Here, we used virus-induced gene silencing (VIGS) to analyze the function of the reported Am-ANT. We then determine the open reading frame (ORF) of Am-ANT and its predicted amino acid sequence. We induced VIGS using Cucumber mosaic virus (CMV-A1) and suppressed the level of Am-ANT mRNA and noted any phenotypic changes. The function of Am-ANT was very similar to that of At-ANT. The A1:ANT-infected Antirrhinum plants had smaller floral organs and leaves, even though cell sizes were unchanged in flowers and larger in leaves. The CMV-based VIGS showed that the cloned Am-ANT gene was indeed functional in cell proliferation and organ growth as observed for At-ANT. In A. majus, CMV vector provide great advantages for analysis of gene functions. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/48722 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 増田 税
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