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Acquired resistance to the rice blast in transgenic rice accumulating the antimicrobial peptide thanatin

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Title: Acquired resistance to the rice blast in transgenic rice accumulating the antimicrobial peptide thanatin
Authors: Imamura, Tomohiro Browse this author
Yasuda, Michiko Browse this author
Kusano, Hiroaki Browse this author
Nakashita, Hideo Browse this author
Ohno, Yuko Browse this author
Kamakura, Takashi Browse this author
Taguchi, Seiichi Browse this author →KAKEN DB
Shimada, Hiroaki Browse this author
Keywords: Antimicrobial peptide
Oryza sativa
Rice blast
Disease resistance
Molecular farming
Issue Date: Jun-2010
Publisher: Springer Netherlands
Journal Title: Transgenic Research
Volume: 19
Issue: 3
Start Page: 415
End Page: 424
Publisher DOI: 10.1007/s11248-009-9320-x
Abstract: Thanatin is an antimicrobial peptide with a strong and wide-ranging antimicrobial spectrum, including certain species of fungi and Gram-negative and -positive bacteria. To evaluate the application of thanatin to the generation of disease-resistant plants, we introduced a synthetic thanatin gene into rice. Several transformants that expressed the introduced gene showed significant level of antimicrobial activity. The substances showing antimicrobial activity were partially purified from these transformants and their properties were determined. The molecule with characteristics similar to those of native thanatin on the elution pattern in HPLC analysis had an identical molecular mass to that of native molecule. It should also be noted that the transformant acquired a sufficient level of resistance to the rice blast fungus, Magnaporthe oryzae, presumably due to the repressive activity of thanatin to its initial stage of infection. This result demonstrates that thanatin has antifungal activity for M. oryzae and that the introduction of the thanatin gene into rice is effective in generating a plant resistant to rice blast disease.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田口 精一

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