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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea
| Title: | Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea |
| Authors: | Atsumi, Go Browse this author | | Nakahara, Kenji S Browse this author →KAKEN DB | | Wada, Tomoko Sugikawa Browse this author | | Choi, Sun Hee Browse this author | | Masuta, Chikara Browse this author →KAKEN DB | | Uyeda, Ichiro Browse this author →KAKEN DB |
| Issue Date: | 2012 |
| Publisher: | Springer |
| Journal Title: | Archives of Virology |
| Volume: | 157 |
| Issue: | 6 |
| Start Page: | 1019 |
| End Page: | 1028 |
| Publisher DOI: | 10.1007/s00705-012-1281-3 |
| PMID: | 22398917 |
| Abstract: | Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/49369 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 中原 健二
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