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Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/49369

Title: Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea
Authors: Atsumi, Go Browse this author
Nakahara, Kenji S Browse this author →KAKEN DB
Wada, Tomoko Sugikawa Browse this author
Choi, Sun Hee Browse this author
Masuta, Chikara Browse this author →KAKEN DB
Uyeda, Ichiro Browse this author →KAKEN DB
Issue Date: 2012
Publisher: Springer
Journal Title: Archives of Virology
Volume: 157
Issue: 6
Start Page: 1019
End Page: 1028
Publisher DOI: 10.1007/s00705-012-1281-3
PMID: 22398917
Abstract: Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.
Type: article (author version)
URI: http://hdl.handle.net/2115/49369
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中原 健二

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