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Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea

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タイトル: Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea
著者: Atsumi, Go 著作を一覧する
Nakahara, Kenji S 著作を一覧する
Wada, Tomoko Sugikawa 著作を一覧する
Choi, Sun Hee 著作を一覧する
Masuta, Chikara 著作を一覧する
Uyeda, Ichiro 著作を一覧する
発行日: 2012年
出版者: Springer
誌名: Archives of Virology
巻: 157
号: 6
開始ページ: 1019
終了ページ: 1028
出版社 DOI: 10.1007/s00705-012-1281-3
抄録: Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/49369
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 中原 健二

 

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