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Xenon-induced inhibition of synchronized bursts in a rat cortical neuronal network

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Title: Xenon-induced inhibition of synchronized bursts in a rat cortical neuronal network
Authors: Uchida, T. Browse this author →KAKEN DB
Suzuki, S. Browse this author
Hirano, Y. Browse this author
Ito, D. Browse this author
Nagayama, M. Browse this author
Gohara, K. Browse this author
Keywords: general anesthesia
multi-electrode array
synapse transmission inhibition
xenon molecule
Issue Date: 12-Jul-2012
Publisher: Elsevier
Journal Title: Neuroscience
Volume: 214
Start Page: 149
End Page: 158
Publisher DOI: 10.1016/j.neuroscience.2012.03.063
PMID: 22531374
Abstract: Xenon (Xe) and other inert gases produce anesthesia via an inhibitory mechanism in neuronal networks. To better understand this mechanism, we measured the electrical signals from cultured rat cortical neuronal networks in a multi-electrode array (MEA) under an applied Xe pressure. We used the MEA to measure the firing of the neuronal network with and without Xe gas pressurized to 0.3 MPa. The MEA system monitored neuronal spikes on 16 electrodes (each 50 x 50 μm2) at a sampling rate of 20 kHz. The embryo rat cortical cells were first cultured on MEAs without Xe for approximately three weeks, at which time they produced synchronized bursts that indicate maturity. Then, with an applied Xe pressure, the synchronized bursts quickly ceased, whereas single spikes continued. The Xe-induced inhibition-recovery of neuronal network firing was reversible: after purging Xe from the system, the synchronized bursts gradually resumed. Thus, Xe did not inhibit single neuron firing, yet reversibly inhibited the synaptic transmission. This finding agrees with the channel-blocker and a modified-hydrate hypothesis of anesthesia, but not the lipid-solubility hypothesis.
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 内田 努

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