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DNA-RNA hybrid formation mediates RNAi-directed heterochromatin formation

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Title: DNA-RNA hybrid formation mediates RNAi-directed heterochromatin formation
Authors: Nakama, Mina Browse this author
Kawakami, Kei Browse this author
Kajitani, Takuya Browse this author
Urano, Takeshi Browse this author
Murakami, Yota Browse this author →KAKEN DB
Keywords: heterochromatin
RNA interference
non-coding RNA
DNA:RNA hybrid
fission yeast
Issue Date: Mar-2012
Publisher: Wiley-Blackwell
Journal Title: Genes to Cells
Volume: 17
Issue: 3
Start Page: 218
End Page: 233
Publisher DOI: 10.1111/j.1365-2443.2012.01583.x
Abstract: Certain non-coding RNAs (ncRNAs) implicated in the regulation of chromatin structure associate with chromatin. During the formation of RNAi-directed heterochromatin in fission yeast, ncRNAs transcribed from heterochromatin are thought to recruit the RNAi machinery to chromatin for the formation of heterochromatin; however, the molecular details of this association are not clear. Here, using RNA-immunoprecipitation assay, we showed that the heterochromatic ncRNA associated with chromatin via the formation of a DNA:RNA hybrid and bound to the RNA-induced transcriptional silencing (RITS) complex. The presence of DNA:RNA hybrid in the cell was also confirmed by immunofluorescence analysis using anti-DNA:RNA hybrid antibody. Overexpression and depletion of RNase H in vivo decreased and increased the amount of DNA:RNA hybrid formed, respectively, and both disturbed heterochromatin. Moreover, DNA:RNA hybrid was formed on, and overexpression of RNase H inhibited the formation of, artificial heterochromatin induced by tethering of RITS to mRNA. These results indicate that heterochromatic ncRNAs are retained on chromatin via the formation of DNA:RNA hybrids and provide a platform for the RNAi-directed heterochromatin assembly and suggest that DNA:RNA hybrid formation plays a role in chromatic ncRNA function.
Rights: The definitive version is available at
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 村上 洋太

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