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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
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DNA-RNA hybrid formation mediates RNAi-directed heterochromatin formation
| Title: | DNA-RNA hybrid formation mediates RNAi-directed heterochromatin formation |
| Authors: | Nakama, Mina Browse this author | | Kawakami, Kei Browse this author | | Kajitani, Takuya Browse this author | | Urano, Takeshi Browse this author | | Murakami, Yota Browse this author →KAKEN DB |
| Keywords: | heterochromatin | | RNA interference | | non-coding RNA | | DNA:RNA hybrid | | fission yeast |
| Issue Date: | Mar-2012 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Genes to Cells |
| Volume: | 17 |
| Issue: | 3 |
| Start Page: | 218 |
| End Page: | 233 |
| Publisher DOI: | 10.1111/j.1365-2443.2012.01583.x |
| Abstract: | Certain non-coding RNAs (ncRNAs) implicated in the regulation of chromatin structure associate with chromatin. During the formation of RNAi-directed heterochromatin in fission yeast, ncRNAs transcribed from heterochromatin are thought to recruit the RNAi machinery to chromatin for the formation of heterochromatin; however, the molecular details of this association are not clear. Here, using RNA-immunoprecipitation assay, we showed that the heterochromatic ncRNA associated with chromatin via the formation of a DNA:RNA hybrid and bound to the RNA-induced transcriptional silencing (RITS) complex. The presence of DNA:RNA hybrid in the cell was also confirmed by immunofluorescence analysis using anti-DNA:RNA hybrid antibody. Overexpression and depletion of RNase H in vivo decreased and increased the amount of DNA:RNA hybrid formed, respectively, and both disturbed heterochromatin. Moreover, DNA:RNA hybrid was formed on, and overexpression of RNase H inhibited the formation of, artificial heterochromatin induced by tethering of RITS to mRNA. These results indicate that heterochromatic ncRNAs are retained on chromatin via the formation of DNA:RNA hybrids and provide a platform for the RNAi-directed heterochromatin assembly and suggest that DNA:RNA hybrid formation plays a role in chromatic ncRNA function. |
| Rights: | The definitive version is available at www3.interscience.wiley.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/49934 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 村上 洋太
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