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Hokkaido University Collection of Scholarly and Academic Papers >
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Difference in transcriptional regulatory function between c-Fos and Fra-2
| Title: | Difference in transcriptional regulatory function between c-Fos and Fra-2 |
| Authors: | Suzuki, Takeshi Browse this author | | Okuno, Hiroyuki Browse this author | | Yoshida, Tetuso Browse this author | | Endo, Toshinori Browse this author | | Nishina, Hiroshi Browse this author | | Iba, Hideo Browse this author |
| Issue Date: | Oct-1991 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic Acids Research |
| Volume: | 19 |
| Issue: | 20 |
| Start Page: | 5537 |
| End Page: | 5542 |
| Publisher DOI: | 10.1093/nar/19.20.5537 |
| Abstract: | Fra-2, one of the Fos-related antigens, is promptly expressed after the growth stimulation of fibroblasts, but its induction peak is later than that of c-Fos. In this report, we examined biochemical properties of Fra-2 and compared them with those of two other Fos family proteins, c-Fos and Fra-1. Like c-Fos and Fra-1, Fra-2 formed stable heterodimers with c-Jun, JunB or JunD in vitro and all these complexes had specific DNAbinding activity to AP-1-binding sites (AP-1 sites) or related sequences. When transiently introduced into a mouse embryonic carcinoma cell line, F9, with reporter genes containing the AP-1 site from the collagenase gene, fra-2 plus c-jun suppressed the transactivation by c-jun alone. This property of Fra-2 is in clear contrast to that of c-Fos, which stimulates the transcriptional activity of c-Jun by forming a stable heterodimer. Analysis of chimeric proteins between c-Fos and Fra-2 indicated that this difference is mainly attributable to their C terminal-half regions. Interestingly, this suppressive effect of Fra-2 was not observed in the combination with JunD: fra-2 plus junD, like c-fos plus junD, had higher transcriptional activity than junD alone. Fra-1 showed essentially the same transcriptional regulatory properties as Fra-2. These differential properties greatly expand the potential range of regulatory functions of the Fos family proteins. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/50026 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 遠藤 俊徳
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