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Difference in transcriptional regulatory function between c-Fos and Fra-2

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Title: Difference in transcriptional regulatory function between c-Fos and Fra-2
Authors: Suzuki, Takeshi Browse this author
Okuno, Hiroyuki Browse this author
Yoshida, Tetuso Browse this author
Endo, Toshinori Browse this author
Nishina, Hiroshi Browse this author
Iba, Hideo Browse this author
Issue Date: Oct-1991
Publisher: Oxford University Press
Journal Title: Nucleic Acids Research
Volume: 19
Issue: 20
Start Page: 5537
End Page: 5542
Publisher DOI: 10.1093/nar/19.20.5537
Abstract: Fra-2, one of the Fos-related antigens, is promptly expressed after the growth stimulation of fibroblasts, but its induction peak is later than that of c-Fos. In this report, we examined biochemical properties of Fra-2 and compared them with those of two other Fos family proteins, c-Fos and Fra-1. Like c-Fos and Fra-1, Fra-2 formed stable heterodimers with c-Jun, JunB or JunD in vitro and all these complexes had specific DNAbinding activity to AP-1-binding sites (AP-1 sites) or related sequences. When transiently introduced into a mouse embryonic carcinoma cell line, F9, with reporter genes containing the AP-1 site from the collagenase gene, fra-2 plus c-jun suppressed the transactivation by c-jun alone. This property of Fra-2 is in clear contrast to that of c-Fos, which stimulates the transcriptional activity of c-Jun by forming a stable heterodimer. Analysis of chimeric proteins between c-Fos and Fra-2 indicated that this difference is mainly attributable to their C terminal-half regions. Interestingly, this suppressive effect of Fra-2 was not observed in the combination with JunD: fra-2 plus junD, like c-fos plus junD, had higher transcriptional activity than junD alone. Fra-1 showed essentially the same transcriptional regulatory properties as Fra-2. These differential properties greatly expand the potential range of regulatory functions of the Fos family proteins.
Type: article
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 遠藤 俊徳

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