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Protein-Protein Interaction Panel Using Mouse Full-Length cDNAs
This item is licensed under:Creative Commons Attribution-NonCommercial 3.0 Unported
| Title: | Protein-Protein Interaction Panel Using Mouse Full-Length cDNAs |
| Authors: | Suzuki, Harukazu Browse this author | | Fukunishi, Yoshifumi Browse this author | | Kagawa, Ikuko Browse this author | | Saito, Rintaro Browse this author | | Oda, Hiroshi Browse this author | | Endo, Toshinori Browse this author | | Kondo, Shinji Browse this author | | Bono, Hidemasa Browse this author | | Okazaki, Yasushi Browse this author | | Hayashizaki, Yoshihide Browse this author |
| Issue Date: | Oct-2001 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Journal Title: | Genome Research |
| Volume: | 11 |
| Issue: | 10 |
| Start Page: | 1758 |
| End Page: | 1765 |
| Publisher DOI: | 10.1101/gr.180101 |
| Abstract: | We have developed a novel assay system for systematic analysis of protein–protein interactions (PPIs) that is characteristic of a PCR-mediated rapid sample preparation and a high-throughput assay system based on the mammalian two-hybrid method. Using gene-specific primers, we successfully constructed the assay samples by two rounds of PCR with up to 3.6 kb from the first-round PCR fragments. In the assay system, we designed all the steps to be performed by adding only samples, reagents, and cells into 384-well assay plates using two types of semiautomatic multiple dispensers. The system enabled us examine more than 20,000 assay wells per day. We detected 145 interactions in our pilot study using 3500 samples derived from mouse full-length enriched cDNAs. Analysis of the interaction data showed both several significant interaction clusters and predicted functions of a few uncharacterized proteins. In combination with our comprehensive mouse full-length cDNA clone bank covering a large part of the whole genes, our high-throughput assay system will discover many interactions to facilitate understanding of the function of uncharacterized proteins and the molecular mechanism of crucial biological processes, and also enable completion of a rough draft of the entire PPI panel in certain cell types or tissues of mouse within a short time. |
| Rights: | http://creativecommons.org/licenses/by-nc/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/50027 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 遠藤 俊徳
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