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Rabring7 Degrades c-Myc through Complex Formation with MM-1
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| Title: | Rabring7 Degrades c-Myc through Complex Formation with MM-1 |
| Authors: | Narita, Rina Browse this author | | Kitaura, Hirotake Browse this author →KAKEN DB | | Torii, Ayako Browse this author | | Tashiro, Erika Browse this author | | Miyazawa, Makoto Browse this author | | Ariga, Hiroyoshi Browse this author →KAKEN DB | | Iguchi-Ariga, Sanae M. M. Browse this author |
| Issue Date: | 23-Jul-2012 |
| Publisher: | Public Library of Science |
| Journal Title: | PLoS One |
| Volume: | 7 |
| Issue: | 7 |
| Start Page: | e41891 |
| Publisher DOI: | 10.1371/journal.pone.0041891 |
| Abstract: | We have reported that a novel c-Myc-binding protein, MM-1, repressed E-box-dependent transcription and transforming activities of c-Myc and that a mutation of A157R in MM-1, which is often observed in patients with leukemia or lymphoma, abrogated all of the repressive activities of MM-1 toward c-Myc, indicating that MM-1 is a novel tumor suppressor. MM-1 also binds to the ubiquitin-proteasome system, leading to degradation of c-Myc. In this study, we identified Rabring7, a Rab7-binding and RING finger-containing protein, as an MM-1-binding protein, and we found that Rabring7 mono-ubiquitinated MM-1 in the cytoplasm without degradation of MM-1. Rabring7 was also found to bind to c-Myc and to ubiquitinate c-Myc in a threonine 58-dependent manner. When c-Myc was co-transfected with MM-1 and Rabring7, c-Myc was degraded. Furthermore, it was found that c-Myc was stabilized in MM-1-knockdown cells even when Rabring7 was transfected and that Rabring7 was bound to and co-localized with MM-1 and c-Myc after MM-1 and Rabring7 had been translocated from the cytoplasm to the nucleus. These results suggest that Rabring7 stimulates c-Myc degradation via mono-ubiquitination of MM-1. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/50059 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 有賀 寛芳
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