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Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate
| Title: | Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate |
| Authors: | Satoh, Yasuharu Browse this author →KAKEN DB | | Tajima, Kenji Browse this author →KAKEN DB | | Nakamoto, Sho Browse this author | | Xuerong, Han Browse this author | | Matsushima, Tokuo Browse this author | | Ohshima, Takeshi Browse this author | | Kawano, Shin Browse this author | | Erata, Tomoki Browse this author →KAKEN DB | | Dairi, Tohru Browse this author →KAKEN DB | | Munekata, Masanobu Browse this author →KAKEN DB |
| Keywords: | biodiesel fuel by-product | | polyhydroxyalkanoate | | pseudomonas | | thermotolerant bacterium |
| Issue Date: | Oct-2011 |
| Publisher: | Blackwell Publishing |
| Journal Title: | Journal of Applied Microbiology |
| Volume: | 111 |
| Issue: | 4 |
| Start Page: | 811 |
| End Page: | 817 |
| Publisher DOI: | 10.1111/j.1365-2672.2011.05093.x |
| PMID: | 21714837 |
| Abstract: | Aims: The aim of the present study was to isolate a thermotolerant microorganism that produces polyhydroxyalkanoates (PHAs) composed of medium-chain-length (mcl)-HA units from a biodiesel fuel (BDF) by-product as a carbon source. Methods and Results: We successfully isolated a thermotolerant microorganism, strain SG4502, capable to accumulate mcl-PHA from a BDF by-product as a carbon source at a cultivation temperature of 45℃. The strain could also produce mcl-PHA from acetate, octanoate, and dodecanoate as sole carbon sources at cultivation temperatures up to 55℃. Taxonomic studies and 16S rRNA gene sequence analysis revealed that strain SG4502 was phylogenetically affiliated with species of the genus Pseudomonas. This study is the first report of PHA synthesis by a thermotolerant Pseudomonas. Conclusions: A novel thermotolerant bacterium capable to accumulate mcl-PHA from a BDF by-product was successfully isolated. Significance and Impact of the Study: A major issue regarding industrial production of microbial PHAs is their much higher production cost compared with conventional petrochemical-based plastic materials. Especially significant are the cost of a fermentative substrate and the running cost to maintain a temperature suitable for microbial growth. Thus, strain SG4502, isolated in this study, which assimilates BDF by-product and produces PHA at high temperature, would be very useful for practical application in industry. |
| Rights: | The definitive version is available at www.blackwell-synergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/50148 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐藤 康治
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