HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >

Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate

Files in This Item:
JAM111-4_811-817.pdf293.88 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/50148

Title: Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate
Authors: Satoh, Yasuharu Browse this author →KAKEN DB
Tajima, Kenji Browse this author →KAKEN DB
Nakamoto, Sho Browse this author
Xuerong, Han Browse this author
Matsushima, Tokuo Browse this author
Ohshima, Takeshi Browse this author
Kawano, Shin Browse this author
Erata, Tomoki Browse this author →KAKEN DB
Dairi, Tohru Browse this author →KAKEN DB
Munekata, Masanobu Browse this author →KAKEN DB
Keywords: biodiesel fuel by-product
polyhydroxyalkanoate
pseudomonas
thermotolerant bacterium
Issue Date: Oct-2011
Publisher: Blackwell Publishing
Journal Title: Journal of Applied Microbiology
Volume: 111
Issue: 4
Start Page: 811
End Page: 817
Publisher DOI: 10.1111/j.1365-2672.2011.05093.x
PMID: 21714837
Abstract: Aims: The aim of the present study was to isolate a thermotolerant microorganism that produces polyhydroxyalkanoates (PHAs) composed of medium-chain-length (mcl)-HA units from a biodiesel fuel (BDF) by-product as a carbon source. Methods and Results: We successfully isolated a thermotolerant microorganism, strain SG4502, capable to accumulate mcl-PHA from a BDF by-product as a carbon source at a cultivation temperature of 45℃. The strain could also produce mcl-PHA from acetate, octanoate, and dodecanoate as sole carbon sources at cultivation temperatures up to 55℃. Taxonomic studies and 16S rRNA gene sequence analysis revealed that strain SG4502 was phylogenetically affiliated with species of the genus Pseudomonas. This study is the first report of PHA synthesis by a thermotolerant Pseudomonas. Conclusions: A novel thermotolerant bacterium capable to accumulate mcl-PHA from a BDF by-product was successfully isolated. Significance and Impact of the Study: A major issue regarding industrial production of microbial PHAs is their much higher production cost compared with conventional petrochemical-based plastic materials. Especially significant are the cost of a fermentative substrate and the running cost to maintain a temperature suitable for microbial growth. Thus, strain SG4502, isolated in this study, which assimilates BDF by-product and produces PHA at high temperature, would be very useful for practical application in industry.
Rights: The definitive version is available at www.blackwell-synergy.com
Type: article (author version)
URI: http://hdl.handle.net/2115/50148
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐藤 康治

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University