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The coupling of synthesis and partitioning of EBV's plasmid replicon is revealed in live cells

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Title: The coupling of synthesis and partitioning of EBV's plasmid replicon is revealed in live cells
Authors: Nanbo, Asuka Browse this author →KAKEN DB
Sugden, Arthur Browse this author
Sugden, Bill Browse this author
Keywords: Epstein-Barr virus
licensed plasmid replicon
partitioning
replication
Issue Date: 3-Oct-2007
Publisher: Nature Publishing Group
Journal Title: The EMBO Journal
Volume: 26
Issue: 19
Start Page: 4252
End Page: 4262
Publisher DOI: 10.1038/sj.emboj.7601853
Abstract: Epstein-Barr virus (EBV) is an exceptionally successful human viral pathogen maintained as a licensed, plasmid replicon in proliferating cells. We have measured the distributions of EBV-derived plasmids in single live cells throughout the cell cycle in the absence of selection and confirmed the measured rates of duplication and partitioning computationally and experimentally. These analyses have uncovered a striking, non-random partitioning for this minimalist plasmid replicon and revealed additional properties of it and its host cells: 1) 84% of the plasmids duplicate during each S phase. 2) All duplicated plasmids are spatially co-localized as pairs, a positioning that is coupled to their non-random partitioning. 3) Each clone of cells requires a certain threshold number of plasmids per cell for its optimal growth under selection. 4) Defects in plasmid synthesis and partitioning are balanced to yield wide distributions of plasmids in clonal populations of cells for which the plasmids provide a selective advantage. These properties of its plasmid replicon underlie EBV's success as a human pathogen.
Type: article (author version)
URI: http://hdl.handle.net/2115/50164
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 南保 明日香

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