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Heat shock protein 47 stress responses in Chinese hamster ovary cells exposed to raw and reclaimed wastewater

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Title: Heat shock protein 47 stress responses in Chinese hamster ovary cells exposed to raw and reclaimed wastewater
Authors: Guizani, Mokhtar Browse this author
Nogoshi, Yosuke Browse this author
Ben Fredj, Fahmi Browse this author
Han, Junkyu Browse this author
Isoda, Hiroko Browse this author
Funamizu, Naoyuki Browse this author →KAKEN DB
Issue Date: Feb-2012
Publisher: Royal Society of Chemistry
Journal Title: Journal of Environmental Monitoring
Volume: 14
Issue: 2
Start Page: 492
End Page: 498
Publisher DOI: 10.1039/c1em10519a
PMID: 22159031
Abstract: As wastewater reclamation and reuse becomes more widespread, risks of exposure to treated wastewater increase. Moreover, an unlimited number of pollutants can be identified in wastewater. Therefore, comprehensive toxicity assessment of treated wastewater is imperative. The objective of this study was to perform a comprehensive toxicity assessment of wastewater treatment systems using stress response bioassays. This powerful tool can comprehensively assess the toxicity of contaminants. In this study, samples from conventional activated sludge treatment, membrane bioreactors (MBRs) with different pore sizes and sludge retention times (SRTs), rapid sand filtration, coagulation, nano-filtration (NF) and reverse osmosis (RO) were investigated. The results of stress response bioassays confirmed that the secondary effluent showed higher stress response than influent indicating that biological treatment generates toxic compounds. The results obtained from molecular weight fractionation of water samples demonstrated that organic matter with a higher molecular weight fraction (>0.1μm) causes toxicity in secondary effluent. Furthermore, supernatant from MBR reactors showed toxicity regardless of SRT. On the other hand, stress response was not detected in MBR permeates except for an MBR equipped with a larger pore size membrane (0.4μm) and with a short SRT (12 days). While rapid sand filtration could not remove the toxic compounds found in secondary effluent, coagulation tests, operated at an appropriate pH, were effective for reducing stress response in the secondary effluent. Experimental findings also showed that stress response was not detected in cases of NF and RO permeate subsequent to MBR treatment.
Rights: J. Environ. Monit., 2012, 14, 492-498 - Reproduced by permission of The Royal Society of Chemistry (RSC)
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: Mokhtar Guizani

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