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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
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Aceticlastic and NaCl-Requiring Methanogen "Methanosaeta pelagica" sp. nov., Isolated from Marine Tidal Flat Sediment
| Title: | Aceticlastic and NaCl-Requiring Methanogen "Methanosaeta pelagica" sp. nov., Isolated from Marine Tidal Flat Sediment |
| Authors: | Mori, Koji Browse this author | | Iino, Takao Browse this author | | Suzuki, Ken-Ichiro Browse this author | | Yamaguchi, Kaoru Browse this author | | Kamagata, Yoichi Browse this author →KAKEN DB |
| Issue Date: | May-2012 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Applied and Environmental Microbiology |
| Volume: | 78 |
| Issue: | 9 |
| Start Page: | 3416 |
| End Page: | 3423 |
| Publisher DOI: | 10.1128/AEM.07484-11 |
| Abstract: | Acetate is a key compound for anaerobic organic matter degradation, and so far, two genera, Methanosaeta and Methanosarcina, are only contributors for acetate degradation among methanogens. An aceticlastic methanogen, designated strain 03d30qT, was isolated from a tidal flat sediment in Futtsu, Japan. The phylogenetic analyses based on 16S rRNA and mcrA genes revealed that the isolate belonged to the genus Methanosaeta, but the optimal Na+ concentration for growth shifted to marine environments unlike the other known Methanosaeta species. The quantitative estimation by using a real-time PCR indicated that the 16S rRNA gene of the genus Methanosaeta was detected in the sediments and the relative abundance ranged from 3.9% to 11.8% of the total archaeal 16S rRNA genes. Also, the amount of the genus Methanosaeta increased with increasing depth and was much higher than that of the genus Methanosarcina. This is the first report of marine Methanosaeta species, and on the basis of phylogenetic and characteristic studies, the novel species is proposed, "Methanosaeta pelagica" sp. nov., with types strain 03d30qT. |
| Rights: | © 2012 by the American Society for Microbiology |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/50384 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鎌形 洋一
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