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A full validated hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin in human plasma ultrafiltrates.

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Title: A full validated hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin in human plasma ultrafiltrates.
Authors: Ito, Hajime Browse this author
Yamaguchi, Hiroaki Browse this author →KAKEN DB
Fujikawa, Asuka Browse this author
Tanaka, Nobuaki Browse this author
Furugen, Ayako Browse this author
Miyamori, Kazuaki Browse this author
Takahashi, Natsuko Browse this author
Ogura, Jiro Browse this author
Kobayashi, Masaki Browse this author →KAKEN DB
Yamada, Takehiro Browse this author
Mano, Nariyasu Browse this author
Iseki, Ken Browse this author →KAKEN DB
Keywords: Oxaliplatin
Hydrophilic interaction liquid chromatography-tandem mass spectrometry
Plasma ultrafiltrates
Issue Date: Dec-2012
Publisher: Elsevier
Journal Title: Journal of pharmaceutical and biomedical analysis
Volume: 71
Start Page: 99
End Page: 103
Publisher DOI: 10.1016/j.jpba.2012.08.010
PMID: 22954448
Abstract: Oxaliplatin is a platinum agent that is used for treatment of colorectal cancer. A sensitive and selective hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin was developed. Human plasma ultrafiltrates were precipitated by acetonitrile containing carboplatin as an internal standard and further diluted with acetonitrile. Chromatographic separation of oxaliplatin and the internal standard was achieved with a column modified with phosphorylcholine and an isocratic mobile phase (acetonitrile/water/acetic acid=90:10:0.1, v/v/v) at the flow rate of 0.2mL/min. The lower limit of quantification for oxaliplatin was 25ng/mL. The linearity range of the method was from 25 to 5000ng/mL. The intra-day precision and inter-day precision (RSD) ranged from 0.8 to 6.1%, and the accuracy (RE) was within ±4.5%. The extraction recoveries from human plasma ultrafiltrates were 83.6-91.6%, and ion suppression caused by matrix components was 86.7-88.5% at three different levels, respectively. This method was applied to a clinical pharmacokinetic study of oxaliplatin in a cancer patient. The maximum concentration of colorectal cancer patient administered oxaliplatin was 1650ng/mL.
Type: article (author version)
URI: http://hdl.handle.net/2115/50389
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 浩明

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