|
Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
A full validated hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin in human plasma ultrafiltrates.
| Title: | A full validated hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin in human plasma ultrafiltrates. |
| Authors: | Ito, Hajime Browse this author | | Yamaguchi, Hiroaki Browse this author →KAKEN DB | | Fujikawa, Asuka Browse this author | | Tanaka, Nobuaki Browse this author | | Furugen, Ayako Browse this author | | Miyamori, Kazuaki Browse this author | | Takahashi, Natsuko Browse this author | | Ogura, Jiro Browse this author | | Kobayashi, Masaki Browse this author →KAKEN DB | | Yamada, Takehiro Browse this author | | Mano, Nariyasu Browse this author | | Iseki, Ken Browse this author →KAKEN DB |
| Keywords: | Oxaliplatin | | Hydrophilic interaction liquid chromatography-tandem mass spectrometry | | Plasma ultrafiltrates |
| Issue Date: | Dec-2012 |
| Publisher: | Elsevier |
| Journal Title: | Journal of pharmaceutical and biomedical analysis |
| Volume: | 71 |
| Start Page: | 99 |
| End Page: | 103 |
| Publisher DOI: | 10.1016/j.jpba.2012.08.010 |
| PMID: | 22954448 |
| Abstract: | Oxaliplatin is a platinum agent that is used for treatment of colorectal cancer. A sensitive and selective hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin was developed. Human plasma ultrafiltrates were precipitated by acetonitrile containing carboplatin as an internal standard and further diluted with acetonitrile. Chromatographic separation of oxaliplatin and the internal standard was achieved with a column modified with phosphorylcholine and an isocratic mobile phase (acetonitrile/water/acetic acid=90:10:0.1, v/v/v) at the flow rate of 0.2mL/min. The lower limit of quantification for oxaliplatin was 25ng/mL. The linearity range of the method was from 25 to 5000ng/mL. The intra-day precision and inter-day precision (RSD) ranged from 0.8 to 6.1%, and the accuracy (RE) was within ±4.5%. The extraction recoveries from human plasma ultrafiltrates were 83.6-91.6%, and ion suppression caused by matrix components was 86.7-88.5% at three different levels, respectively. This method was applied to a clinical pharmacokinetic study of oxaliplatin in a cancer patient. The maximum concentration of colorectal cancer patient administered oxaliplatin was 1650ng/mL. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/50389 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 山口 浩明
|
