| Title: | A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study. |
| Authors: | Yamaguchi, Hiroaki Browse this author →KAKEN DB |
| Fujikawa, Asuka Browse this author |
| Ito, Hajime Browse this author |
| Tanaka, Nobuaki Browse this author |
| Furugen, Ayako Browse this author |
| Miyamori, Kazuaki Browse this author |
| Takahashi, Natsuko Browse this author |
| Ogura, Jiro Browse this author |
| Kobayashi, Masaki Browse this author →KAKEN DB |
| Yamada, Takehiro Browse this author |
| Mano, Nariyasu Browse this author |
| Iseki, Ken Browse this author →KAKEN DB |
| Keywords: | Docetaxel |
| LC/ESI-MS/MS |
| Rapid and sensitive analysis |
| Issue Date: | 15-Apr-2012 |
| Publisher: | Elsevier |
| Journal Title: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| Volume: | 893-894 |
| Start Page: | 157 |
| End Page: | 161 |
| Publisher DOI: | 10.1016/j.jchromb.2012.02.003 |
| PMID: | 22429876 |
| Abstract: | Docetaxel is a taxane family antineoplastic agent widely employed in cancer chemotherapy. We developed a liquid chromatography/tandem mass spectrometry method for the determination of docetaxel in human plasma. Plasma samples were deproteinized by acetonitrile containing internal standard paclitaxel. Chromatographic separation was performed on a TSKgel ODS-100 V 3 μm (50 mm × 2.0 mm i.d.) column using a mobile phase composed of acetonitrile-methanol-water-formic acid (50:5:45:0.1, v/v/v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. This method covered a linearity range of 5-5000 ng/mL with the lower limit of quantification of 5 ng/mL. The intra-day precision and inter-day precision (R.S.D.) of analysis were less than 6.7%, and the accuracy (R.E.) was within ± 9.0% at the concentrations of 5, 20, 200, and 2000 ng/mL. The total run time was 5.0 min. This method was successfully applied for clinical pharmacokinetic investigation. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/50395 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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