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A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study.

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/50395

Title: A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study.
Authors: Yamaguchi, Hiroaki Browse this author →KAKEN DB
Fujikawa, Asuka Browse this author
Ito, Hajime Browse this author
Tanaka, Nobuaki Browse this author
Furugen, Ayako Browse this author
Miyamori, Kazuaki Browse this author
Takahashi, Natsuko Browse this author
Ogura, Jiro Browse this author
Kobayashi, Masaki Browse this author →KAKEN DB
Yamada, Takehiro Browse this author
Mano, Nariyasu Browse this author
Iseki, Ken Browse this author →KAKEN DB
Keywords: Docetaxel
LC/ESI-MS/MS
Rapid and sensitive analysis
Issue Date: 15-Apr-2012
Publisher: Elsevier
Journal Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume: 893-894
Start Page: 157
End Page: 161
Publisher DOI: 10.1016/j.jchromb.2012.02.003
PMID: 22429876
Abstract: Docetaxel is a taxane family antineoplastic agent widely employed in cancer chemotherapy. We developed a liquid chromatography/tandem mass spectrometry method for the determination of docetaxel in human plasma. Plasma samples were deproteinized by acetonitrile containing internal standard paclitaxel. Chromatographic separation was performed on a TSKgel ODS-100 V 3 μm (50 mm × 2.0 mm i.d.) column using a mobile phase composed of acetonitrile-methanol-water-formic acid (50:5:45:0.1, v/v/v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. This method covered a linearity range of 5-5000 ng/mL with the lower limit of quantification of 5 ng/mL. The intra-day precision and inter-day precision (R.S.D.) of analysis were less than 6.7%, and the accuracy (R.E.) was within ± 9.0% at the concentrations of 5, 20, 200, and 2000 ng/mL. The total run time was 5.0 min. This method was successfully applied for clinical pharmacokinetic investigation.
Type: article (author version)
URI: http://hdl.handle.net/2115/50395
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 浩明

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