| Title: | Quantification of intracellular and extracellular prostanoids stimulated by A23187 by liquid chromatography/electrospray ionization tandem mass spectrometry |
| Authors: | Furugen, Ayako Browse this author |
| Yamaguchi, Hiroaki Browse this author →KAKEN DB |
| Tanaka, Nobuaki Browse this author |
| Ito, Hajime Browse this author |
| Miyamori, Kazuaki Browse this author |
| Fujikawa, Asuka Browse this author |
| Takahashi, Natsuko Browse this author |
| Ogura, Jiro Browse this author |
| Kobayashi, Masaki Browse this author →KAKEN DB |
| Yamada, Takehiro Browse this author |
| Mano, Nariyasu Browse this author |
| Iseki, Ken Browse this author →KAKEN DB |
| Keywords: | Prostanoid |
| LC/MS/MS |
| Intracellular measurement |
| Issue Date: | 15-Nov-2011 |
| Publisher: | Elsevier |
| Journal Title: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| Volume: | 879 |
| Issue: | 30 |
| Start Page: | 3378 |
| End Page: | 3385 |
| Publisher DOI: | 10.1016/j.jchromb.2011.09.003 |
| PMID: | 21963481 |
| Abstract: | Prostanoids are bioactive substances that contribute to various biological and pathological processes. To evaluate both extracellular and intracellular levels of prostanoids at the same time, we developed methods for quantification of extracellular and intracellular levels of prostanoids, including prostaglandin E(2) (PGE(2)), PGD(2), PGD(2 alpha), 6-keto PGF(1 alpha), and TXB(2), in cultured cells using liquid chromatography/tandem mass spectrometry (LC/MS/MS), and we validated the LC/MS/MS methods. A solid-phase extraction cartridge was used for extraction of prostanoids. The prostanoids were separated by a C(18) column with an isocratic flow of acetonitrile/water/acetic acid (40:60:0.1, v/v/v). Calibration curves of extracellular measurement for the prostanoids were linear in the range from 0.1 to 100 ng/mL (r(2) > 0.999), and those of intracellular measurement were linear in the range from 0.05 to 50 ng (r(2) > 0.999). Validation assessment showed that both methods of extracellular and intracellular measurements were highly reliable with good accuracy and precision. We also applied the methods to human airway epithelial Calu-3 cells and human lung adenocarcinoma epithelial A549 cells. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/50396 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
