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Quantification of intracellular and extracellular prostanoids stimulated by A23187 by liquid chromatography/electrospray ionization tandem mass spectrometry

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Title: Quantification of intracellular and extracellular prostanoids stimulated by A23187 by liquid chromatography/electrospray ionization tandem mass spectrometry
Authors: Furugen, Ayako Browse this author
Yamaguchi, Hiroaki Browse this author →KAKEN DB
Tanaka, Nobuaki Browse this author
Ito, Hajime Browse this author
Miyamori, Kazuaki Browse this author
Fujikawa, Asuka Browse this author
Takahashi, Natsuko Browse this author
Ogura, Jiro Browse this author
Kobayashi, Masaki Browse this author →KAKEN DB
Yamada, Takehiro Browse this author
Mano, Nariyasu Browse this author
Iseki, Ken Browse this author →KAKEN DB
Keywords: Prostanoid
LC/MS/MS
Intracellular measurement
Issue Date: 15-Nov-2011
Publisher: Elsevier
Journal Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume: 879
Issue: 30
Start Page: 3378
End Page: 3385
Publisher DOI: 10.1016/j.jchromb.2011.09.003
PMID: 21963481
Abstract: Prostanoids are bioactive substances that contribute to various biological and pathological processes. To evaluate both extracellular and intracellular levels of prostanoids at the same time, we developed methods for quantification of extracellular and intracellular levels of prostanoids, including prostaglandin E(2) (PGE(2)), PGD(2), PGD(2 alpha), 6-keto PGF(1 alpha), and TXB(2), in cultured cells using liquid chromatography/tandem mass spectrometry (LC/MS/MS), and we validated the LC/MS/MS methods. A solid-phase extraction cartridge was used for extraction of prostanoids. The prostanoids were separated by a C(18) column with an isocratic flow of acetonitrile/water/acetic acid (40:60:0.1, v/v/v). Calibration curves of extracellular measurement for the prostanoids were linear in the range from 0.1 to 100 ng/mL (r(2) > 0.999), and those of intracellular measurement were linear in the range from 0.05 to 50 ng (r(2) > 0.999). Validation assessment showed that both methods of extracellular and intracellular measurements were highly reliable with good accuracy and precision. We also applied the methods to human airway epithelial Calu-3 cells and human lung adenocarcinoma epithelial A549 cells.
Type: article (author version)
URI: http://hdl.handle.net/2115/50396
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 浩明

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