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Establishment of an efficient in vitro culture and particle bombardment-mediated transformation systems in Miscanthus sinensis Anderss., a potential bioenergy crop

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Title: Establishment of an efficient in vitro culture and particle bombardment-mediated transformation systems in Miscanthus sinensis Anderss., a potential bioenergy crop
Authors: WANG, XUN Browse this author
YAMADA, TETSUYA Browse this author
KONG, FAN-JIANG Browse this author
ABE, YUKI Browse this author
HOSHINO, YOICHIRO Browse this author →KAKEN DB
SATO, HIROKO Browse this author
TAKAMIZO, TADASHI Browse this author
KANAZAWA, AKIRA Browse this author →KAKEN DB
YAMADA, TOSHIHIKO Browse this author →KAKEN DB
Keywords: callus induction
Miscanthus sinensis
particle bombardment
selection regime
Issue Date: Aug-2011
Publisher: Wiley-Blackwell
Journal Title: Global Change Biology Bioenergy
Volume: 3
Issue: 4
Start Page: 322
End Page: 332
Publisher DOI: 10.1111/j.1757-1707.2011.01090.x
Abstract: Plants belonging to the genus Miscanthus are considered promising bioenergy crops. Here, we report the establishment of tissue culture system through particle bombardment- mediated transformation in Miscanthus sinensis Anderss. Callus was induced efficiently from mature seeds in a medium containing a combination of a relatively high level of 2,4-dichlorophenoxyacetic acid (5mgL 1) and a relatively low level of 6-benzyladenine (BA) (0.01mgL 1). Callus induction potential of 18 accessions of M. sinensis, which were collected from various sites in Japan, was compared. Significant correlation was detected between compact (embryogenic) callus induction frequency and average annual air temperature in collection sites. An accession from Tanegashima Island showed the highest production of compact callus. We found that a higher level of BA causes callus browning; the 2mg L 1 BA is the optimal concentration for regeneration. Both compact and friable calli were suitable for particle bombardment transformation. Through selection under the presence of 50mgL 1 hygromycin for 3 weeks and further selection under the presence of 150mgL 1 for 1 month, hygromycin-resistant calli survived, of which 72.2% had been entirely transformed. Plants were regenerated from calli in the presence of hygromycin; transcripts of the hpt and gfp genes, which were cobombarded to the calli, were detected in the regenerated plants. This is the first report on the establishment of the in vitro culture of M. sinensis using mature seeds, the variation of callus formation among accessions collected from various sites in Japan, and particle bombardment-mediated transformation in the genus Miscanthus.
Rights: This is the pre-peer-reviewed version of the following article: GCB Bioenergy. 3(4):322-332, 2011.8., which has been published in final form at
Type: article (author version)
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 敏彦

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