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A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/50782

Title: A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine
Authors: Koizumi, Nobuo Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Harunari, Tsunehito Browse this author
Tanikawa, Tsutomu Browse this author
Tokiwa, Toshihiro Browse this author
Uchimura, Eriko Browse this author
Furuya, Tokujiro Browse this author
Mingala, Claro Niegos Browse this author
Villanueva, Marvin Ardeza Browse this author
Ohnishi, Makoto Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Issue Date: Jun-2012
Publisher: American Society for Microbiology
Journal Title: Journal of Clinical Microbiology
Volume: 50
Issue: 6
Start Page: 2072
End Page: 2074
Publisher DOI: 10.1128/JCM.00481-12
Abstract: We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene, of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB-nested PCR.
Rights: © 2012 American Society for Microbiology
Type: article (author version)
URI: http://hdl.handle.net/2115/50782
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 定彦

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