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A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine
| Title: | A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine |
| Authors: | Koizumi, Nobuo Browse this author | | Nakajima, Chie Browse this author →KAKEN DB | | Harunari, Tsunehito Browse this author | | Tanikawa, Tsutomu Browse this author | | Tokiwa, Toshihiro Browse this author | | Uchimura, Eriko Browse this author | | Furuya, Tokujiro Browse this author | | Mingala, Claro Niegos Browse this author | | Villanueva, Marvin Ardeza Browse this author | | Ohnishi, Makoto Browse this author | | Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Issue Date: | Jun-2012 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Journal of Clinical Microbiology |
| Volume: | 50 |
| Issue: | 6 |
| Start Page: | 2072 |
| End Page: | 2074 |
| Publisher DOI: | 10.1128/JCM.00481-12 |
| Abstract: | We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene, of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB-nested PCR. |
| Rights: | © 2012 American Society for Microbiology |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/50782 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 定彦
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