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The effects of heat induction and the siRNA biogenesis pathway on the transgenerational transposition of ONSEN, a copia-like retrotransposon in Arabidopsis thaliana

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Title: The effects of heat induction and the siRNA biogenesis pathway on the transgenerational transposition of ONSEN, a copia-like retrotransposon in Arabidopsis thaliana
Authors: Matsunaga, Wataru Browse this author
Kobayashi, Akie Browse this author
Kato, Atsushi Browse this author →KAKEN DB
Ito, Hidetaka Browse this author →KAKEN DB
Keywords: siRNA
Issue Date: May-2012
Publisher: Oxford University Press
Journal Title: Plant and Cell Physiology
Volume: 53
Issue: 5
Start Page: 824
End Page: 833
Publisher DOI: 10.1093/pcp/pcr179
PMID: 22173101
Abstract: Environmental stress influences genetic and epigenetic regulation in plant genomes. We previously reported that heat stress activated a copia-like retrotransposon named ONSEN (Ito et al., 2011). To investigate the heat sensitivity and transgenerational activation of ONSEN, we analyzed the stress response by temperature shift and multi-treatments of heat stress. ONSEN was activated at 37℃, and the newly inserted ONSEN was transcriptionally activate and mobile to the next generation subjected to heat stress, indicating that the regulation of ONSEN is independent from positional effects on the chromosome. Reciprocal crosses with activated ONSEN revealed that the transgenerational transposition was inherited from both sexes, indicating that the transposition is suppressed independent of gametophytic regulation. We showed previously that ONSEN was transposed in mutants deficient in siRNA biogenesis, including nrpd2 and rdr2, but not dcl3. To define the functional redundancy of DCL proteins in Arabidopsis, we analyzed ONSEN activation in mutants deficient in Dicer-like proteins, including dcl2, dcl3 and dcl4. ONSEN was nearly immobile in a single Dicer mutant; however, some transgenerational transpositions were observed in dcl2/dcl3/dcl4 triple mutants subjected to heat stress. This indicated that the Dicer family is redundant for ONSEN transposition. To examine the activation of ONSEN in undifferentiated cells, ONSEN transcripts and synthesized DNA were analyzed in heat-stressed callus tissue. In contrast with vegetative tissue, high accumulation of the transcripts and amplified DNA copies of ONSEN were detected in callus. This result indicated that ONSEN activation is controlled by cell-specific regulatory mechanisms.
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version Plant Cell Physiol (2012) 53 (5): 824-833 is available online at:
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 秀臣

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