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Title: ラット心筋細胞の凍結保存におけるジメチルスルホキシドの有効性評価
Other Titles: Evaluation of Viability of Cryopreserved Rat Cardiac Myocytes and Effects of Dimethyl Sulfoxide Concentration on Cryopreservation
Authors: 宮村, 謙一郎1 Browse this author
永山, 昌史2 Browse this author →KAKEN DB
郷原, 一寿3 Browse this author →KAKEN DB
平, 敏夫4 Browse this author
清水, 恭子5 Browse this author
酒井, 雅人6 Browse this author
内田, 努7 Browse this author →KAKEN DB
Authors(alt): Miyamura, Kenichiro1
Nagayama, Masafumi2
Gohara, Kazutoshi3
Taira, Toshio4
Shimizu, Kyoko5
Sakai, Masato6
Uchida, Tsutomu7
Issue Date: 15-Oct-2010
Publisher: 低温生物工学会
Journal Title: 低温生物工学会誌
Journal Title(alt): Cryobiology and Cryotechnology
Volume: 56
Issue: 2
Start Page: 111
End Page: 117
Abstract: Cryopreserved cardiac myocytes are expected to be useful for the development of new drug against heart disease. However, the appropriate cryopreservation process for cardiac myocytes has not been established yet. Therefore, in this study, we evaluate the effectiveness of dimethyl sulfoxide (DMSO) on the cryopreservation of cardiac myocytes by three measurements: 1) the total viability (including cardiac myocytes) just after thawing with trypan blue staining, 2) the observation of morphology of cardiac myocytes for 7 days culture by real-time phase contrast microscopy and immunofluorescent staining, and 3) the variation of their beating rates for 7 days after thawing. The total viabilities of cryopreserved cells with 3 v/v% to 20 v/v% DMSO concentration were higher than those without DMSO. Thawed cells contracted regularly and spontaneously during culture, as did the non-cryopreserved cells. The immunofluorescence observation indicated that the thawed cardiac myocytes expressed α-actinin. Therefore, cardiac myocytes could be survived by the cryopreservation with DMSO concentrations between 3 v/v% and 20 v/v%, which had the normal contraction rates during culture for 7 days.
Rights: 利用は著作権の範囲内に限られる.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 内田 努

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