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Dimethyl sulfoxideによる分散ラット心筋細胞の凍結保護機構の実験的検討

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Title: Dimethyl sulfoxideによる分散ラット心筋細胞の凍結保護機構の実験的検討
Other Titles: Experimental Investigations of Cryopreservation Mechanisms on Dispersed Rat Cardiac Myocytes with Dimethyl Sulfoxide
Authors: 内田, 努1 Browse this author →KAKEN DB
宮村, 謙一郎2 Browse this author
永山, 昌史3 Browse this author →KAKEN DB
郷原, 一寿4 Browse this author →KAKEN DB
Authors(alt): Uchida, Tsutomu1
Miyamura, Kenichiro2
Nagayama, Masafumi3
Gohara, Kazutoshi4
Issue Date: 15-Apr-2012
Publisher: 低温生物工学会
Journal Title: 低温生物工学会誌
Journal Title(alt): Cryobiology and cryotechnology
Volume: 58
Issue: 1
Start Page: 59
End Page: 63
Abstract: Dimethyl sulfoxide (DMSO) is one of the useful cryoprotective agents for a variety of cells and tissues allowing prolonged storage at subzero temperatures. Since most of the optimum DMSO concentrations for the different cells and tissues cryopreservations are similar, we predict the reason for the optimum DMSO concentration by measuring the thermodynamic properties of culture medium including DMSO and by observing the toxicity of DMSO on the neonatal rat cardiac myocytes. The melting point measurements suggest that the larger DMSO concentration medium has lower melting points, which would prevent the cell damage by ice reformation during the thawing process. However, such medium has higher toxic effect on the cryopreserved cells. These two contrary factors can predict the optimum concentration of DMSO in the medium for the cryopreservation of cells including the cardiac myocytes.
Rights: 利用は著作権の範囲内に限られる.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 内田 努

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