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タイトル: ホスホリパーゼA2による高度不飽和脂肪酸含有ホスファチジルコリンの合成と細胞分化誘導作用に及ぼす影響
その他のタイトル: Synthesis of Phosphatidylcholine Containing Highly Unsaturated Fatty Acid by Phospholipase A2 and Effect on Retinoic Acid Induced Differentiation of HL-60 Cells
著者: 細川, 雅史 著作を一覧する
大島, 宏哲 著作を一覧する
甲野, 裕之 著作を一覧する
高橋, 是太郎 著作を一覧する
羽田野, 六男 著作を一覧する
小田島, 粛夫 著作を一覧する
発行日: 1993年
出版者: 日本水産学会
誌名: 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries
巻: 59
号: 2
開始ページ: 309
終了ページ: 314
出版社 DOI: 10.2331/suisan.59.309
抄録: Phosphatidylcholine containing highly unsaturated fatty acid (HUFA-PC) was prepared by porcine pancreatic phospholipase A2, which catalyzed esterification between lysophosphatidylcholine (LPC) and highly unsaturated fatty acid (HUFA), under a scaled-up reaction system. Fatty acid mixture prepared from sardine oil, purified eicosapentaenoic acid (EPA), and purified docosahexaenoic acid (DHA) were used as the substrates of HUFA. The yield of HUFA-PC was 17.0-19.9%. Synthesized phosphatidylcholine containing DHA (DHA-PC) prepared from LPC and DHA had a DHA content of 46.5%, which indicated almost the maximum theoretical incorporation rate. DHA-PC molecular species consisted of (16:0, DHA), (18:0, DHA), (18:1, DHA), (18:2, DHA), and (18:3, DHA). It was obvious that the acyl groups in sn-1 of these molecular species originated from the substrate LPC. The rate of retinoic acid induced differentiation of the human promyelocytic leukemia cell line (HL-60) was promoted by growing HL-60 cells in 10% FBS-PNM pre-treated with either EPA-PC or DHA-PC.
Rights: ©1993 公益社団法人日本水産学会 The Japanese Society of Fisheries Science
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高橋 是太郎


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