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Title: ホスホリパーゼA2による高度不飽和脂肪酸含有ホスファチジルコリンの合成と細胞分化誘導作用に及ぼす影響
Other Titles: Synthesis of Phosphatidylcholine Containing Highly Unsaturated Fatty Acid by Phospholipase A2 and Effect on Retinoic Acid Induced Differentiation of HL-60 Cells
Authors: 細川, 雅史1 Browse this author →KAKEN DB
大島, 宏哲2 Browse this author
甲野, 裕之3 Browse this author
高橋, 是太郎4 Browse this author →KAKEN DB
羽田野, 六男5 Browse this author →KAKEN DB
小田島, 粛夫6 Browse this author
Authors(alt): Hosokawa, Masashi1
Ohshima, Hiroaki2
Kohno, Hiroyuki3
Takahashi, Koretaro4
Hatano, Mutsuo5
Odashima, Shizuo6
Issue Date: 1993
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Journal Title(alt): Bulletin of the Japanese Society of Scientific Fisheries
Volume: 59
Issue: 2
Start Page: 309
End Page: 314
Publisher DOI: 10.2331/suisan.59.309
Abstract: Phosphatidylcholine containing highly unsaturated fatty acid (HUFA-PC) was prepared by porcine pancreatic phospholipase A2, which catalyzed esterification between lysophosphatidylcholine (LPC) and highly unsaturated fatty acid (HUFA), under a scaled-up reaction system. Fatty acid mixture prepared from sardine oil, purified eicosapentaenoic acid (EPA), and purified docosahexaenoic acid (DHA) were used as the substrates of HUFA. The yield of HUFA-PC was 17.0-19.9%. Synthesized phosphatidylcholine containing DHA (DHA-PC) prepared from LPC and DHA had a DHA content of 46.5%, which indicated almost the maximum theoretical incorporation rate. DHA-PC molecular species consisted of (16:0, DHA), (18:0, DHA), (18:1, DHA), (18:2, DHA), and (18:3, DHA). It was obvious that the acyl groups in sn-1 of these molecular species originated from the substrate LPC. The rate of retinoic acid induced differentiation of the human promyelocytic leukemia cell line (HL-60) was promoted by growing HL-60 cells in 10% FBS-PNM pre-treated with either EPA-PC or DHA-PC.
Rights: © 1993 公益社団法人日本水産学会
© 1993 The Japanese Society of Fisheries Science
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 是太郎

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