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Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production
| Title: | Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production |
| Authors: | Song, Yuyang Browse this author | | Matsumoto, Ken'ichiro Browse this author →KAKEN DB | | Yamada, Miwa Browse this author | | Gohda, Aoi Browse this author | | Brigham, Christopher J. Browse this author | | Sinskey, Anthony J. Browse this author | | Taguchi, Seiichi Browse this author →KAKEN DB |
| Keywords: | Polylactide | | Biobased plastic | | PHA synthase | | Polyhydroxyalkanoate | | Polyhydroxybutyrate |
| Issue Date: | Mar-2012 |
| Publisher: | Springer-Verlag |
| Journal Title: | Applied Microbiology and Biotechnology |
| Volume: | 93 |
| Issue: | 5 |
| Start Page: | 1917 |
| End Page: | 1925 |
| Publisher DOI: | 10.1007/s00253-011-3718-0 |
| PMID: | 22127753 |
| Abstract: | The first biosynthetic system for lactate (LA)-based polyesters was previously created in recombinant Escherichia coli. Here, we have begun efforts to upgrade the prototype polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C. glutamicum to generate monomer substrates, lactyl-CoA (LA-CoA) and 3-hydroxybutyryl-CoA (3HB-CoA), for the copolymerization catalyzed by the LA-polymerizing enzyme (LPE). LA-CoA was synthesized by D-lactate dehydrogenase and propionyl-CoA transferase, while 3HB-CoA was supplied by β-ketothiolase (PhaA) and NADPH-dependent acetoacetyl-CoA reductase (PhaB). The functional expression of these enzymes led to a production of P(LA-co-3HB) with high LA fractions (96.8 mol%). The omission of PhaA and PhaB from this pathway led to a further increase in LA fraction up to 99.3 mol%. The newly engineered C. glutamicum potentially serves as a food-grade and biomedically applicable platform for the production of poly(lactic acid)-like polyester. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52101 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松本 謙一郎
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