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Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production

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Title: Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production
Authors: Song, Yuyang Browse this author
Matsumoto, Ken'ichiro Browse this author →KAKEN DB
Yamada, Miwa Browse this author
Gohda, Aoi Browse this author
Brigham, Christopher J. Browse this author
Sinskey, Anthony J. Browse this author
Taguchi, Seiichi Browse this author →KAKEN DB
Keywords: Polylactide
Biobased plastic
PHA synthase
Issue Date: Mar-2012
Publisher: Springer-Verlag
Journal Title: Applied Microbiology and Biotechnology
Volume: 93
Issue: 5
Start Page: 1917
End Page: 1925
Publisher DOI: 10.1007/s00253-011-3718-0
PMID: 22127753
Abstract: The first biosynthetic system for lactate (LA)-based polyesters was previously created in recombinant Escherichia coli. Here, we have begun efforts to upgrade the prototype polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C. glutamicum to generate monomer substrates, lactyl-CoA (LA-CoA) and 3-hydroxybutyryl-CoA (3HB-CoA), for the copolymerization catalyzed by the LA-polymerizing enzyme (LPE). LA-CoA was synthesized by D-lactate dehydrogenase and propionyl-CoA transferase, while 3HB-CoA was supplied by β-ketothiolase (PhaA) and NADPH-dependent acetoacetyl-CoA reductase (PhaB). The functional expression of these enzymes led to a production of P(LA-co-3HB) with high LA fractions (96.8 mol%). The omission of PhaA and PhaB from this pathway led to a further increase in LA fraction up to 99.3 mol%. The newly engineered C. glutamicum potentially serves as a food-grade and biomedically applicable platform for the production of poly(lactic acid)-like polyester.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松本 謙一郎

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