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Effects of endogenous proteins and microRNA target sequence in a positive feedback system.
| Title: | Effects of endogenous proteins and microRNA target sequence in a positive feedback system. |
| Authors: | Kanda, Genki N Browse this author | | Togashi, Ryohei Browse this author | | Harashima, Hideyoshi Browse this author →KAKEN DB | | Kamiya, Hiroyuki Browse this author →KAKEN DB |
| Keywords: | positive feedback system | | secreted alkaline phosphatase | | transcription factor | | microRNA | | long-term transgene expression |
| Issue Date: | Sep-2012 |
| Publisher: | Pharmaceutical Society of Japan |
| Journal Title: | Biological & pharmaceutical bulletin |
| Volume: | 35 |
| Issue: | 9 |
| Start Page: | 1534 |
| End Page: | 1538 |
| Publisher DOI: | 10.1248/bpb.b12-00329 |
| PMID: | 22975505 |
| Abstract: | A positive feedback system, using GAL4-vp16 (a fusion protein of yeast GAL4 and herpes simplex virus vp16) as an activator and firefly luciferase as a reporter, maintained luciferase expression for 7 d in mice. However, the luciferase expression decreased after 7 d, and this phenomenon could be caused by immunoreactions against these exogenous proteins. This hypothesis was examined by the following three strategies, designed to avoid the putative immunoreactions: (i) use of the endogenous secreted alkaline phosphatase (SEAP) protein as a reporter, (ii) replacement of vp16 with endogenous transcription factors, and (iii) insertion of the target sequence of microRNA expressed in cells of hematopoietic origin, to suppress GAL4-vp16 expression in antigen-presenting cells. The results obtained in this study suggested that silencing would be induced by mechanism(s) besides immunoreactions against reporter and activator proteins. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/52122 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 紙谷 浩之
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