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Effects of endogenous proteins and microRNA target sequence in a positive feedback system.

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Title: Effects of endogenous proteins and microRNA target sequence in a positive feedback system.
Authors: Kanda, Genki N Browse this author
Togashi, Ryohei Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Kamiya, Hiroyuki Browse this author →KAKEN DB
Keywords: positive feedback system
secreted alkaline phosphatase
transcription factor
long-term transgene expression
Issue Date: Sep-2012
Publisher: Pharmaceutical Society of Japan
Journal Title: Biological & pharmaceutical bulletin
Volume: 35
Issue: 9
Start Page: 1534
End Page: 1538
Publisher DOI: 10.1248/bpb.b12-00329
PMID: 22975505
Abstract: A positive feedback system, using GAL4-vp16 (a fusion protein of yeast GAL4 and herpes simplex virus vp16) as an activator and firefly luciferase as a reporter, maintained luciferase expression for 7 d in mice. However, the luciferase expression decreased after 7 d, and this phenomenon could be caused by immunoreactions against these exogenous proteins. This hypothesis was examined by the following three strategies, designed to avoid the putative immunoreactions: (i) use of the endogenous secreted alkaline phosphatase (SEAP) protein as a reporter, (ii) replacement of vp16 with endogenous transcription factors, and (iii) insertion of the target sequence of microRNA expressed in cells of hematopoietic origin, to suppress GAL4-vp16 expression in antigen-presenting cells. The results obtained in this study suggested that silencing would be induced by mechanism(s) besides immunoreactions against reporter and activator proteins.
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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