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Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids
| Title: | Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids |
| Authors: | Obara, Keisuke Browse this author →KAKEN DB | | Kojima, Ryo Browse this author | | Kihara, Akio Browse this author →KAKEN DB |
| Keywords: | membrane traffic | | multivesicular body | | Rab | | GTPase | | yeast | | Very long-chain fatty acid | | vesicular transport | | endosome |
| Issue Date: | Mar-2013 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Journal Title: | Journal of Lipid Research |
| Volume: | 54 |
| Issue: | 3 |
| Start Page: | 831 |
| End Page: | 842 |
| Publisher DOI: | 10.1194/jlr.M034678 |
| Abstract: | Very long-chain fatty acids (VLCFAs), fatty acids with chain length of > 20, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the presented study, we screened for multicopy suppressors that rescued temperature-sensitive growth of VLCFA-limited yeast cells, and identified the VPS21 gene, encoding a Rab GTPase, as such a suppressor. When the vps21Δ mutation was introduced into a deletion mutant of the SUR4 gene, which encodes a VLCFA elongase, a synthetic growth defect was observed. Endosome-mediated vesicular trafficking pathways, including endocytosis and the CPY pathway, were severely impaired in sur4Δ vps21Δ double mutants, while the AP-3 pathway that bypasses the endosome was unaffected. In addition, the sur4Δ mutant also exhibited a synthetic growth defect when combined with the deletion of VPS3, which encodes a subunit of the CORVET (class C core vacuole/endosome tethering) complex that tethers transport vesicles to the late endosome/multivesicular body (MVB). These results suggest that requirement of VLCFAs is especially high in the endosomal pathways, of all the intracellular trafficking pathways. |
| Rights: | Copyright © 2013 the American Society for Biochemistry and Molecular Biology |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52255 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木原 章雄
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