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Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids

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Title: Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids
Authors: Obara, Keisuke Browse this author →KAKEN DB
Kojima, Ryo Browse this author
Kihara, Akio Browse this author →KAKEN DB
Keywords: membrane traffic
multivesicular body
Very long-chain fatty acid
vesicular transport
Issue Date: Mar-2013
Publisher: American Society for Biochemistry and Molecular Biology
Journal Title: Journal of Lipid Research
Volume: 54
Issue: 3
Start Page: 831
End Page: 842
Publisher DOI: 10.1194/jlr.M034678
Abstract: Very long-chain fatty acids (VLCFAs), fatty acids with chain length of > 20, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the presented study, we screened for multicopy suppressors that rescued temperature-sensitive growth of VLCFA-limited yeast cells, and identified the VPS21 gene, encoding a Rab GTPase, as such a suppressor. When the vps21Δ mutation was introduced into a deletion mutant of the SUR4 gene, which encodes a VLCFA elongase, a synthetic growth defect was observed. Endosome-mediated vesicular trafficking pathways, including endocytosis and the CPY pathway, were severely impaired in sur4Δ vps21Δ double mutants, while the AP-3 pathway that bypasses the endosome was unaffected. In addition, the sur4Δ mutant also exhibited a synthetic growth defect when combined with the deletion of VPS3, which encodes a subunit of the CORVET (class C core vacuole/endosome tethering) complex that tethers transport vesicles to the late endosome/multivesicular body (MVB). These results suggest that requirement of VLCFAs is especially high in the endosomal pathways, of all the intracellular trafficking pathways.
Rights: Copyright © 2013 the American Society for Biochemistry and Molecular Biology
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木原 章雄

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