|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Molecular cloning and characterization of the corticoid receptors from the American alligator
| Title: | Molecular cloning and characterization of the corticoid receptors from the American alligator |
| Authors: | Oka, Kaori Browse this author | | Kohno, Satomi Browse this author | | Urushitani, Hiroshi Browse this author | | Guillette, Louis J., Jr. Browse this author | | Ohta, Yasuhiko Browse this author | | Iguchi, Taisen Browse this author | | Katsu, Yoshinao Browse this author →KAKEN DB |
| Keywords: | Alligator | | Glucocorticoid receptor | | Mineralocorticoid receptor | | Cloning | | Transactivation |
| Issue Date: | 30-Jan-2013 |
| Publisher: | Elsevier Ireland |
| Journal Title: | Molecular and Cellular Endocrinology |
| Volume: | 365 |
| Issue: | 2 |
| Start Page: | 153 |
| End Page: | 161 |
| Publisher DOI: | 10.1016/j.mce.2012.10.014 |
| PMID: | 23127802 |
| Abstract: | Steroid hormones are essential for health in vertebrates. Corticosteroids, for example, have a regulatory role in many physiological functions, such as osmoregulation, respiration, immune responses, stress responses, reproduction, growth, and metabolism. Although extensively studied in mammals and some non-mammalian species, the molecular mechanisms of corticosteroid hormone (glucocorticoids and mineralocorticoids) action are poorly understood in reptiles. Here, we have evaluated hormone receptor-ligand interactions in the American alligator (Alligator mississippiensis), following the isolation of cDNAs encoding a glucocorticoid receptor (GR) and a mineralocorticoid receptor (MR). The full-length alligator GR (aGR) and aMR cDNAs were obtained using 5' and 3' rapid amplification cDNA ends (RACE). The deduced amino acid sequences exhibited high identity to the chicken orthologs (aGR: 83%; aMR: 90%). Using transient transfection assays of mammalian cells, both aGR and aMR proteins displayed corticosteroid-dependent activation of transcription from keto-steroid hormone responsive, murine mammary tumor virus promoters. We further compared the ligand-specifity of human, chicken, Xenopus, and zebrafish GR and MR. We found that the alligator and chicken GR/MR have very similar amino acid sequences, and this translates to very similar ligand specificity. This is the first report of the full-coding regions of a reptilian GR and MR, and the examination of their transactivation by steroid hormones. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52257 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 勝 義直
|
