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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Analysis of salivary gland transcripts of the sand fly Lutzomyia ayacuchensis, a vector of Andean-type cutaneous leishmaniasis
| Title: | Analysis of salivary gland transcripts of the sand fly Lutzomyia ayacuchensis, a vector of Andean-type cutaneous leishmaniasis |
| Authors: | Kato, Hirotomo Browse this author →KAKEN DB | | Jochim, Ryan C. Browse this author | | Gomez, Eduardo A. Browse this author | | Uezato, Hiroshi Browse this author →KAKEN DB | | Mimori, Tatsuyuki Browse this author →KAKEN DB | | Korenaga, Masataka Browse this author | | Sakurai, Tatsuya Browse this author | | Katakura, Ken Browse this author →KAKEN DB | | Valenzuela, Jesus G. Browse this author | | Hashiguchi, Yoshihisa Browse this author →KAKEN DB |
| Keywords: | Lutzomyia ayacuchensis | | Salivary gland | | Transcript | | Bioinformatics | | cDNA library |
| Issue Date: | Jan-2013 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Infection, Genetics and Evolution |
| Volume: | 13 |
| Start Page: | 56 |
| End Page: | 66 |
| Publisher DOI: | 10.1016/j.meegid.2012.08.024 |
| Abstract: | The saliva of blood sucking insects contains potent pharmacologically active components that assist them in counteracting the host hemostatic and inflammatory systems during blood feeding. In addition, sand fly salivary proteins affect host immunity and have the potential to be a vaccine against Leishmania infection. In the present study, the salivary gland transcripts of Lutzomyia (Lu.) ayacuchensis, a vector of cutaneous leishmaniasis in Ecuadorian and Peruvian Andes, were analyzed by sequencing randomly selected clones of the salivary gland cDNA library of this sand fly. This resulted in the identification of the most abundant transcripts coding for secreted proteins. These proteins were homologous to the salivary molecules present in other sand flies including the RGD-containing peptide, PpSP15/SL1 family protein, yellow-related protein, putative apyrase, antigen 5-related protein, D7 family protein, and 27 kDa salivary protein. Of note, homologues of maxadilan, an active vasodilator abundantly present in saliva of Lu. longipalpis, were not identified. This analysis is the first description of salivary proteins from a sand fly of the subgenus Helcocyrtomyia and from vector of cutaneous leishmaniasis in the New World. The present analysis will provide further insights into the evolution of salivary components in blood sucking arthropods. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52263 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加藤 大智
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