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Knockdown of legumain inhibits cleavage of annexin A2 in the mouse kidney

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Title: Knockdown of legumain inhibits cleavage of annexin A2 in the mouse kidney
Authors: Yamane, Takuya Browse this author
Hachisu, Rei Browse this author
Yuguchi, Motoki Browse this author
Takeuchi, Keisuke Browse this author
Murao, Sato Browse this author
Yamamoto, Yoshio Browse this author
Ogita, Hisakazu Browse this author
Takasawa, Toshihide Browse this author
Ohkubo, Iwao Browse this author
Ariga, Hiroyoshi Browse this author →KAKEN DB
Keywords: Legumain
Annexin A2
Cationic liposome
siRNA
In vivo
Mouse kidney
Issue Date: 11-Jan-2013
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Journal Title: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume: 430
Issue: 2
Start Page: 482
End Page: 487
Publisher DOI: 10.1016/j.bbrc.2012.12.010
PMID: 23237799
Abstract: Legumain (EC 3.4.22.34) is an asparaginyl endopeptidase. Strong legumain activity was observed in the mouse kidney, and legumain was highly expressed in tumors. We previously reported that bovine kidney annexin A2 was co-purified with legumain and that legumain cleaved the N-terminal region of annexin A2 at an Asn residue in vitro. In this study, to determine whether annexin A2 is cleaved by legumain in vivo, siRNA-lipoplex targeting mouse legumain was injected into mouse tail veins. Mouse kidneys were then isolated and the effect of knockdown of legumain expression on annexin A2 cleavage was examined. The results showed that both legumain mRNA and protein expression levels were decreased in the siRNA-treated mouse kidneys and that legumain activity toward a synthetic substrate, Z-Ala-Ala-Asn-MCA, was decreased by about 40% in the kidney but not in the liver or spleen. Furthermore, cleavage of annexin A2 at the N-terminal region was decreased in the mouse kidney that had been treated with the legumain siRNA-lipoplex. These results suggest that legumain siRNA was delivered to the kidney by using LipoTrust and that the reduced legumain expression inhibited legumain-induced degradation of annexin A2 in vivo. (C) 2012 Elsevier Inc. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/52625
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山根 拓也

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