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Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme
| Title: | Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme |
| Authors: | Nduko, John Masani Browse this author | | Matsumoto, Ken'ichiro Browse this author →KAKEN DB | | Ooi, Toshihiko Browse this author →KAKEN DB | | Taguchi, Seiichi Browse this author →KAKEN DB |
| Keywords: | Lignocellulose | | Bio-based plastic | | Polylactic acid | | Microbial cell factory |
| Issue Date: | Jan-2013 |
| Publisher: | ELSEVIER |
| Journal Title: | METABOLIC ENGINEERING |
| Volume: | 15 |
| Start Page: | 159 |
| End Page: | 166 |
| Publisher DOI: | 10.1016/j.ymben.2012.11.007 |
| PMID: | 23202750 |
| Abstract: | Xylose, which is a major constituent of lignocellulosic biomass, was utilized for the production of poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)], having transparent and flexible properties. The recombinant Escherichia coil JW0885 (pflA(-)) expressing LA-polymerizing enzyme (LPE) and monomer supplying enzymes grown on xylose produced a copolymer having a higher LA fraction (34 mol%) than that grown on glucose (26 mol%). This benefit of xylose was further enhanced by combining it with an evolved LPE (ST/FS/QK), achieving a copolymer production having 60 mol% LA from xylose, while glucose gave a 47 mol% LA under the same condition. The overall carbon yields from the sugars to the polymer were similar for xylose and glucose, but the ratio of the LA and 3HB units in the copolymer was different. Notably, the P(LA-co-3HB) yield from xylose (7.3 g l(-1)) was remarkably higher than that of P(3HB) (4.1 g l(-1)), indicating P(LA-co-3HB) as a potent target for xylose utilization. (C) 2012 Elsevier Inc. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52731 |
| Appears in Collections: | 総合化学院 (Graduate School of Chemical Sciences and Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: Nduko John Masani
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