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Biosynthetic assembly of cytochalasin backbone

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Title: Biosynthetic assembly of cytochalasin backbone
Authors: Fujii, Ryuya Browse this author
Minami, Atsushi Browse this author
Gomi, Katsuya Browse this author
Oikawa, Hideaki Browse this author →KAKEN DB
Keywords: Cytochalasin
Biosynthesis
Polyketide synthase
Non-ribosomal peptide synthetase
Fungal metabolite
Issue Date: 5-Jun-2013
Publisher: Elsevier
Journal Title: Tetrahedron Letters
Volume: 54
Issue: 23
Start Page: 2999
End Page: 3002
Publisher DOI: 10.1016/j.tetlet.2013.03.120
Abstract: Cytochalasins are an important class of fungal natural products in view of structural diversity and biological activities. Although their biosynthetic studies have been examined extensively, the detailed molecular assembly mechanism remains to be solved. We have succeeded to heterologously express the cytochalasin polyketide synthase-non-ribosomal peptide synthetase (PKS-NRPS) hybrid gene ccsA and the trans-acting enoyl-CoA reductase gene ccsC in Aspergillus oryzae. The resultant transformant produced a novel metabolite possessing the cytochalasin backbone. This established that CcsA is capable of constructing the octaketide connected with phenylalanine in collaboration with CcsC, and that CcsA R domain catalyzes reductive cleavage of the thio-tethered PKS-NRPS product. (C) 2013 Elsevier Ltd. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/52893
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 及川 英秋

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