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Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization

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Title: Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization
Authors: Kiboku, Takayuki Browse this author
Katoh, Tsuyoshi Browse this author
Nakamura, Akio Browse this author
Kitamura, Akira Browse this author
Kinjo, Masataka Browse this author →KAKEN DB
Murakami, Yota Browse this author →KAKEN DB
Takahashi, Masayuki Browse this author →KAKEN DB
Keywords: myosin II
conformational change
cell migration
Issue Date: Feb-2013
Publisher: Wiley-Blackwell
Journal Title: Genes to Cells
Volume: 18
Issue: 2
Start Page: 90
End Page: 109
Publisher DOI: 10.1111/gtc.12021
PMID: 23237600
Abstract: Nonmuscle myosin II forms a folded conformation (10S form) in the inactivated state; however, the physiological importance of the 10S form is still unclear. To investigate the role of 10S form, we generated a chimeric mutant of nonmuscle myosin IIB (IIB-SK1・2), in which S1462-R1490 and L1551-E1577 were replaced with the corresponding portions of skeletal muscle myosin heavy chain. The IIB-SK1・2 mutant did not fold into a 10S form under physiological condition in vitro. IIB-SK1・2 was less dynamic by stabilizing the filamentous form and accumulated in the posterior region of migrating cells. IIB-SK1・2 functioned properly in cytokinesis, but altered migratory properties; the rate and directional persistence were increased by IIB-SK1・2 expression. Surprisingly, endogenous nonmuscle myosin IIA was excluded from the posterior region of migrating cells expressing IIB-SK1・2, which may underlie the change of the cellular migratory properties. These results suggest that the 10S form is necessary for maintaining nonmuscle myosin II in an unassembled state and for recruitment of nonmuscle myosin II to a specific region of the cell.
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Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 正行

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