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Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization
| Title: | Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization |
| Authors: | Kiboku, Takayuki Browse this author | | Katoh, Tsuyoshi Browse this author | | Nakamura, Akio Browse this author | | Kitamura, Akira Browse this author | | Kinjo, Masataka Browse this author →KAKEN DB | | Murakami, Yota Browse this author →KAKEN DB | | Takahashi, Masayuki Browse this author →KAKEN DB |
| Keywords: | myosin II | | nonmuscle | | conformational change | | cell migration | | cytokinesis |
| Issue Date: | Feb-2013 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Genes to Cells |
| Volume: | 18 |
| Issue: | 2 |
| Start Page: | 90 |
| End Page: | 109 |
| Publisher DOI: | 10.1111/gtc.12021 |
| PMID: | 23237600 |
| Abstract: | Nonmuscle myosin II forms a folded conformation (10S form) in the inactivated state; however, the physiological importance of the 10S form is still unclear. To investigate the role of 10S form, we generated a chimeric mutant of nonmuscle myosin IIB (IIB-SK1・2), in which S1462-R1490 and L1551-E1577 were replaced with the corresponding portions of skeletal muscle myosin heavy chain. The IIB-SK1・2 mutant did not fold into a 10S form under physiological condition in vitro. IIB-SK1・2 was less dynamic by stabilizing the filamentous form and accumulated in the posterior region of migrating cells. IIB-SK1・2 functioned properly in cytokinesis, but altered migratory properties; the rate and directional persistence were increased by IIB-SK1・2 expression. Surprisingly, endogenous nonmuscle myosin IIA was excluded from the posterior region of migrating cells expressing IIB-SK1・2, which may underlie the change of the cellular migratory properties. These results suggest that the 10S form is necessary for maintaining nonmuscle myosin II in an unassembled state and for recruitment of nonmuscle myosin II to a specific region of the cell. |
| Rights: | The definitive version is available at www3.interscience.wiley.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53054 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高橋 正行
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