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Substrate specificity, plasma membrane localization, and lipid modification of the aldehyde dehydrogenase ALDH3B1

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Title: Substrate specificity, plasma membrane localization, and lipid modification of the aldehyde dehydrogenase ALDH3B1
Authors: Kitamura, Takuya Browse this author
Naganuma, Tatsuro Browse this author
Abe, Kensuke Browse this author
Nakahara, Kanae Browse this author
Ohno, Yusuke Browse this author
Kihara, Akio Browse this author →KAKEN DB
Keywords: Aldehyde
Aldehyde dehydrogenase
Sphingolipid
Plasma membrane
Prenylation
Palmitoylation
Issue Date: Aug-2013
Publisher: Elsevier Science BV
Journal Title: Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids
Volume: 1831
Issue: 8
Start Page: 1395
End Page: 1401
Publisher DOI: 10.1016/j.bbalip.2013.05.007
PMID: 23721920
Abstract: The accumulation of reactive aldehydes is implicated in the development of several disorders. Aldehyde dehydrogenases (ALDHs) detoxify aldehydes by oxidizing them to the corresponding carboxylic acids. Among the 19 human ALDHs, ALDH3A2 is the only known ALDH that catalyzes the oxidation of long-chain fatty aldehydes including C16 aldehydes (hexadecanal and trans-2-hexadecenal) generated through sphingolipid metabolism. In the present study, we have identified that ALDH3B1 is also active in vitro toward C16 aldehydes and demonstrated that overexpression of ALDH3B1 restores the sphingolipid metabolism in the ALDH3A2-deficient cells. In addition, we have determined that ALDH3B1 is localized in the plasma membrane through its C-terminal dual lipidation (palmitoylation and prenylation) and shown that the prenylation is required particularly for the activity toward hexadecanal. Since knockdown of ALDH3B1 does not cause further impairment of the sphingolipid metabolism in the ALDH3A2-deficient cells, the likely physiological function of ALDH3B1 is to oxidize lipid-derived aldehydes generated in the plasma membrane and not to be involved in the sphingolipid metabolism in the endoplasmic reticulum. (C) 2013 Elsevier B.V. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/53089
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木原 章雄

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