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Identification of the recognition sequence and target proteins for DJ-1 protease

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Title: Identification of the recognition sequence and target proteins for DJ-1 protease
Authors: Mitsugi, Hitomi Browse this author
Niki, Takeshi Browse this author →KAKEN DB
Takahashi-Niki, Kazuko Browse this author →KAKEN DB
Tanimura, Kyoko Browse this author
Yoshizawa-Kumagaye, Kumiko Browse this author
Tsunemi, Masahiko Browse this author
Iguchi-Ariga, Sanae M. M. Browse this author →KAKEN DB
Ariga, Hiroyoshi Browse this author →KAKEN DB
Keywords: DJ-1
Issue Date: 19-Aug-2013
Publisher: Elsevier science bv
Journal Title: Febs letters
Volume: 587
Issue: 16
Start Page: 2493
End Page: 2499
Publisher DOI: 10.1016/j.febslet.2013.06.032
PMID: 23831022
Abstract: DJ-1, the product of familial Parkinson's disease gene and an oncogene, is a cysteine protease which plays a role in anti-oxidative stress reaction. In this study, we identified the recognition sequence for DJ-1 protease by using recombinant DJ-1 and a peptide library. Protease activity of DJ-1 lacking C-terminal alpha-helix (DJ-1 Delta H9) was stronger than that of full-sized DJ-1, and the most susceptible sequence digested by DJ-1 Delta H9 was valine-lysine-valine-alanine (VKVA) under the optimal conditions of pH 5.5 and 0 mM NaCl. Divalent ions, especially Cu2+, were inhibitory to DJ-1's protease activity. c-abl oncogene 1 product (ABL1) and kinesin family member 1B (KIF1B) containing VKVA were digested by DJ-1 Delta H9. Structured summary of protein interactions: DJ-1 cleaves IUF1B by enzymatic study (View interaction) DJ-1 cleaves ABLI by enzymatic study (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 有賀 寛芳

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