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Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3
| Title: | Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3 |
| Authors: | Kimoto, Mai Browse this author | | Kitagawa, Tsuyuki Browse this author | | Kobayashi, Isao Browse this author | | Nakata, Tomohiro Browse this author | | Kuroiwa, Asato Browse this author →KAKEN DB | | Takiya, Shigeharu Browse this author →KAKEN DB |
| Keywords: | Bombyx mori | | Sericin-1 | | Silk gene | | Silk gland | | Homeodomain | | Gene gun |
| Issue Date: | Nov-2012 |
| Publisher: | Springer-Verlag |
| Journal Title: | Development Genes and Evolution |
| Volume: | 222 |
| Issue: | 6 |
| Start Page: | 351 |
| End Page: | 359 |
| Publisher DOI: | 10.1007/s00427-012-0418-4 |
| PMID: | 23070540 |
| Abstract: | The sericin-1 gene encoding a glue protein is expressed in the middle silk gland (MSG) of the silkworm, Bombyx mori. A member of the class III POU domain transcription factors, POU-M1, was cloned as the factor bound to the SC site of the sericin-1 promoter and has been proposed to be a positive transcription factor. In this study, we analyzed the expression pattern of the POU-M1 gene in fourth and fifth instars in comparison with the pattern of the sericin-1 gene. The POU-M1 gene was expressed strongly in the region anterior to the sericin-1-expressing portion of the silk gland at both feeding stages. As the sericin-1-expressing region expands from the posterior to middle portions of the MSG in the fifth instar, the POU-M1-expressing region retreated from the middle to anterior portion. Introduction of the expression vector of POU-M1 into the silk glands by gene gun technology repressed promoter activity of the sericin-1 gene, suggesting that POU-M1 regulates the sericin-1 gene negatively. An in vitro binding assay showed that POU-M1 bound not only to the SC site but also to other promoter elements newly detected in vivo. Another spatiotemporal specific factor MIC binds to these elements, and POU-M1 competed with MIC to bind at the -70 site essential for promoter activity. These results suggest that POU-M1 is involved in restricting the anterior boundary of the sericin-1-expressing region in the silk gland by inhibiting the binding of the transcriptional activator to the promoter elements. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53467 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 滝谷 重治
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