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Cyclin B1 mRNA translation is temporally controlled through formation and disassembly of RNA granules

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Title: Cyclin B1 mRNA translation is temporally controlled through formation and disassembly of RNA granules
Authors: Kotani, Tomoya Browse this author →KAKEN DB
Yasuda, Kyota Browse this author
Ota, Ryoma Browse this author
Yamashita, Masakane Browse this author →KAKEN DB
Issue Date: 30-Sep-2013
Publisher: Rockefeller univ press
Journal Title: Journal of cell biology
Volume: 202
Issue: 7
Start Page: 1041
End Page: 1055
Publisher DOI: 10.1083/jcb.201302139
Abstract: Temporal control of messenger RNA (mRNA) translation is an important mechanism for regulating cellular, neuronal, and developmental processes. However, mechanisms that coordinate timing of translational activation remain largely unresolved. Full-grown oocytes arrest meiosis at prophase I and deposit dormant mRNAs. Of these, translational control of cyclin B1 mRNA in response to maturation-inducing hormone is important for normal progression of oocyte maturation, through which oocytes acquire fertility. In this study, we found that dormant cyclin B1 mRNA forms granules in the cytoplasm of zebrafish and mouse oocytes. Real-time imaging of translation revealed that the granules disassemble at the time of translational activation during maturation. Formation of cyclin B1 RNA granules requires binding of the mRNA to Pumilio1 protein and depends on actin filaments. Disruption of cyclin B1 RNA granules accelerated the timing of their translational activation after induction of maturation, whereas stabilization hindered translational activation. Thus, our results suggest that RNA granule formation is critical for the regulation of timing of translational activation.
Rights: ©Kotani et al., 2013. Originally published in Journal of cell biology. doi: 10.1083/jcb.201302139
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小谷 友也

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