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Targeted gene delivery in the cricket brain, using in vivo electroporation

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Title: Targeted gene delivery in the cricket brain, using in vivo electroporation
Authors: Matsumoto, Chihiro Sato Browse this author
Shidara, Hisashi Browse this author
Matsuda, Koji Browse this author
Nakamura, Taro Browse this author
Mito, Taro Browse this author
Matsumoto, Yukihisa Browse this author
Oka, Kotaro Browse this author
Ogawa, Hiroto Browse this author →KAKEN DB
Keywords: Insect
Green fluorescent protein
Issue Date: Dec-2013
Journal Title: Journal of Insect Physiology
Volume: 59
Issue: 12
Start Page: 1235
End Page: 1241
Publisher DOI: 10.1016/j.jinsphys.2013.10.001
PMID: 24161373
Abstract: The cricket (Gryllus bimaculatus) is a hemimetabolous insect that is emerging as a model organism for the study of neural and molecular mechanisms of behavioral traits. However, research strategies have been limited by a lack of genetic manipulation techniques that target the nervous system of the cricket. The development of a new method for efficient gene delivery into cricket brains, using in vivo electroporation, is described here. Plasmid DNA, which contained an enhanced green fluorescent protein (eGFP) gene, under the control of a G. bimaculatus actin (Gb′-act) promoter, was injected into adult cricket brains. Injection was followed by electroporation at a sufficient voltage. Expression of eGFP was observed within the brain tissue. Localized gene expression, targeted to specific regions of the brain, was also achieved using a combination of local DNA injection and fine arrangement of the electroporation electrodes. Further studies using this technique will lead to a better understanding of the neural and molecular mechanisms that underlie cricket behaviors.
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小川 宏人

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