|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
Oxidative stress induction of DJ-1 protein in reactive astrocytes scavenges free radicals and reduces cell injury.
| Title: | Oxidative stress induction of DJ-1 protein in reactive astrocytes scavenges free radicals and reduces cell injury. |
| Authors: | Yanagida, Takashi Browse this author | | Tsushima, Jun Browse this author | | Kitamura, Yoshihisa Browse this author →KAKEN DB | | Yanagisawa, Daijiro Browse this author | | Takata, Kazuyuki Browse this author →KAKEN DB | | Shibaike, Tomonori Browse this author | | Yamamoto, Atsuko Browse this author | | Taniguchi, Takashi Browse this author →KAKEN DB | | Yasui, Hiroyuki Browse this author →KAKEN DB | | Taira, Takahiro Browse this author →KAKEN DB | | Morikawa, Shigehiro Browse this author →KAKEN DB | | Inubushi, Toshihiro Browse this author | | Tooyama, Ikuo Browse this author →KAKEN DB | | Ariga, Hiroyoshi Browse this author →KAKEN DB |
| Keywords: | DJ-1 | | release | | astrocytes | | focal ischemia | | oxidative stress sensor | | neuroprotection |
| Issue Date: | Jan-2009 |
| Publisher: | Hindawi Pub. |
| Journal Title: | Oxidative medicine and cellular longevity |
| Volume: | 2 |
| Issue: | 1 |
| Start Page: | 36 |
| End Page: | 42 |
| Publisher DOI: | 10.4161/oxim.2.1.7985 |
| PMID: | 20046643 |
| Abstract: | Astrocytes, one of the predominant types of glial cells, function as both supportive and metabolic cells for the brain. Under cerebral ischemia/reperfusion-induced oxidative conditions, astrocytes accumulate and activate in the ischemic region. DJ-1 has recently been shown to be a sensor of oxidative stress in living cells. However, the function of astrocytic DJ-1 is still unknown. In the present study, to clarify the effect of astrocytic DJ-1 protein under massive oxidative insult, we used a focal ischemic rat model that had been subjected to middle cerebral artery occlusion (MCAO) and reperfusion. We then investigated changes in the distribution of DJ-1 in astrocytes, DJ-1 release from cultured astrocytes, and the effects of recombinant DJ-1 protein on hydrogen peroxide (H(2)O(2))-induced death in normal and DJ-1-knockdown SH-SY5Y cells and on in vitro scavenging of hydroxyl radicals ((*)OH) by electron spin resonance spectrometry. At 24 h after 2-h MCAO and reperfusion, an infarct lesion was markedly observed using magnetic resonance imaging and 2,3,5-triphenyltetrazolium chloride staining. In addition, reactive astrocytes enhanced DJ-1 expression in the penumbral zone of the ischemic core and that DJ-1 protein was extracellularly released from astrocytes by H2O2 in in vitro primary cultures. Although DJ-1-knockdown SH-SY5Y cells were markedly vulnerable to oxidative stress, treatment with glutathione S-transferase-tagged recombinant human DJ-1 protein (GST-DJ-1) significantly inhibited H(2)O(2)-induced cell death. In addition, GST-DJ-1 protein directly scavenged (*)OH. These results suggest that oxidative stress induces the release of astrocytic DJ-1 protein, which may contribute to astrocyte-mediated neuroprotection. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53702 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 有賀 寛芳
|
